Photoperiodic Adjustments in Immune Function Protect Siberian Hamsters from Lethal Endotoxemia

• Published in: Journal of biological rhythms 2003-02, Vol.18 (1), p.51-62
• Main Authors: Prendergast, Brian J., Hotchkiss, Andrew K., Bilbo, Staci D., Kinsey, Steven G., Nelson, Randy J.
• Format: Article
• Language: English
• Subjects: Animals; Body Weight - physiology; Cell Division - drug effects; Cricetinae; Cytokines - biosynthesis; Endotoxins - toxicity; Female; Immune system; Immunity, Cellular - drug effects; Immunity, Cellular - physiology; Lethal Dose 50; Lymphocytes - drug effects; Lymphocytes - physiology; Macrophages - drug effects; Macrophages - metabolism; Macrophages - physiology; Male; Organ Size - physiology; Phodopus; Phodopus sungorus; Photoperiod; Rodents; Seasons; Sex Characteristics; Shock, Septic - immunology; Shock, Septic - physiopathology; Spleen - chemistry; Spleen - drug effects; Survival Analysis; Testis - drug effects; Tumor Necrosis Factor-alpha - metabolism
• ISSN: 0748-7304; 1552-4531
• DOI: 10.1177/0748730402239676

Seasonal changes in day length enhance or suppress components of immune function in individuals of several mammalian species. Siberian hamsters (Phodopus sungorus) exhibit multiple changes in neuroendocrine, reproductive, and immune function after exposure to short days. The manner in which these changes are integrated into the host response to pathogens is not well understood. The present experiments tested the hypothesis that short-day changes in immune function alter the pathogenesis of septic shock and survival after challenge with endotoxin. Male and female Siberian hamsters raised in long-day photoperiods were transferred as adults to short days or remained in their natal photoperiod. Six to 8 weeks later, hamsters were injected i.p. with 0, 1, 2.5, 10, 25, or 50 mg/kg bacterial lipopolysaccharide (LPS) (the biologically active constituent of endotoxin), and survival was monitored for 96 h. Short days significantly improved survival of male hamsters treated with 10 or 25 mg/kg LPS and improved survival in females treated with 50 mg/kg LPS. Transfer from long to short days shifted the LD5 0 in males by approximately 90%, from 5.3 to 9.9 mg/kg, and in females from 11.1 to 15.0 mg/kg ([#002B]35%). Long-day females were more resistant than were males to lethal endotoxemia. In vitro production of the proinflammatory cytokine TNFα in response to LPS stimulation was significantly lower in macrophages extracted from short-day relative to long-day hamsters, as were circulating concentrations of TNFα in vivo after i.p. administration of LPS, suggesting that diminished cytokine responses to LPS in short days may mitigate the lethality of endotoxemia. Adaptation to short days induces changes in immune parameters that affect survival in the face of immune challenges.