Studies on the sensitivity of Spirulina platensis to antibiotics and herbicide: relationship with selectable markers for genetic transformation

The cyanobacterium Spirulina platensis was tested for sensitivity to 6 antibiotics: kanamycin (Km), neomycin (Neo), geneticin (G418), ampicillin (Ap), chloramphenicol (Cm), hygromycin (Hm) and to 1 herbicide: L-phosphinothricin (PPT), all of which are frequently applied as selectable markers in plan...

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Bibliographic Details
Published in:Bioresource technology 1999-10, Vol.70 (1), p.89-93
Main Authors: Cao, Jixiang, Xu, Zengfu, Qiu, Guohua, Li, Baojian
Format: Article
Language:English
Subjects:
Algae
Antibiotics
Biological and medical sciences
Biomarkers
Biotechnology
Fundamental and applied biological sciences. Psychology
Gene transfer
Genetic engineering
genetic markers
Genetic technics
glufosinate
Herbicide
Herbicides
Methods. Procedures. Technologies
Plant cell culture
Selectable markers
Sensitivity
Spirulina platensis
Vectors (cloning, transfer, expression). Insertion sequences and transposons
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Summary:The cyanobacterium Spirulina platensis was tested for sensitivity to 6 antibiotics: kanamycin (Km), neomycin (Neo), geneticin (G418), ampicillin (Ap), chloramphenicol (Cm), hygromycin (Hm) and to 1 herbicide: L-phosphinothricin (PPT), all of which are frequently applied as selectable markers in plant gene engineering. Growth tests, carried out at different concentrations of the agents above, showed that S. platensis was sensitive to Hm, Cm and PPT, while highly resistant to Km, Neo, G418 and Ap, indicating that Hm, Cm and PPT were potent inhibitors of S. platensis growth and able to be employed as selectable markers for further transformation studies for this alga.
ISSN:0960-8524
1873-2976
DOI:10.1016/S0960-8524(99)00002-4