Recovery of proteins from biomass grown in pig manure microalgae-based treatment plants by alkaline hydrolysis and acidic precipitation

•Protein recovery by pH-shift from a consortium microalgae-bacteria biomass.•ANOVA of NaOH concentration, temperature and time effect on process parameters.•Intermediate alkaline concentration (0.5 M NaOH) provided maxima extraction yields.•High solubilisation by alkaline hydrolysis but low acidic p...

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Bibliographic Details
Published in:Bioresource technology 2019-02, Vol.273, p.599-607
Main Authors: Lorenzo-Hernando, Ana, Ruiz-Vegas, Javier, Vega-Alegre, Marisol, Bolado-Rodríguez, Silvia
Format: Article
Language:English
Subjects:
Acids
Alkalies
Amino Acids - metabolism
Animals
Biomass
Bioreactors
Extraction
Fatty Acids - metabolism
Hydrogen-Ion Concentration
Hydrolysis
Lipid Metabolism
Lipids
Manure
Microalgae - metabolism
pH-shift
Protein Biosynthesis
Proteins - metabolism
Scenedesmus almeriensis
Solubilisation
Swine
Temperature
Wastewater
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Summary:•Protein recovery by pH-shift from a consortium microalgae-bacteria biomass.•ANOVA of NaOH concentration, temperature and time effect on process parameters.•Intermediate alkaline concentration (0.5 M NaOH) provided maxima extraction yields.•High solubilisation by alkaline hydrolysis but low acidic precipitation efficiency.•Co-extraction of lipids increased with process intensity. The influence of three variables on key parameters of the protein extraction process (an alkaline hydrolysis followed by an acidic precipitation) for biomass from innovative photo-bioreactors for pig manure treatment was evaluated. Alkaline hydrolysis provided high solubilisation values (up to 66.5% of the biomass), augmenting with increasing values of the three studied variables (NaOH concentration, temperature and time). Nevertheless, moderate total (13.2%) and protein extraction yields (16.9%) were obtained, which was attributable to protein denaturation or to the low effectivity of the precipitation method. Extracts rich in proteins (53.5%–77.9%) with suitable amino acid profiles were obtained, but significant amounts of the initial lipids (up to 44.6%) were co-extracted probably due to fatty acids saponification. These results establish the first step for future studies in enhancing cell wall disruption and protein recovery by coupling alkaline hydrolysis with other mechanical pre-treatments, while considering alternative separation and purification methods.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2018.11.068