Leptin stimulates DMP-1 and DSPP expression in human dental pulp via MAPK 1/3 and PI3K signaling pathways

•Leptin stimulates DSPP expression via PI3K pathway in human dental pulp.•Leptin stimulates DMP-1 synthesis via MAPK pathway in human dental pulp.•DSSP and DMP-1 are non-collagenous proteins implicated in dentinogenesis.•These results support the role of leptin in pulpal reparative response.•The use...

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Published in:Archives of oral biology 2019-02, Vol.98, p.126-131
Main Authors: Martín-González, Jenifer, Pérez-Pérez, Antonio, Cabanillas-Balsera, Daniel, Vilariño-García, Teresa, Sánchez-Margalet, Victor, Segura-Egea, Juan José
Format: Article
Language:English
Subjects:
Dental pulp
Dental pulp reparative response
Dentinogenesis
Dentistry
DMP-1
DSPP
IP3K
JAK-STAT
Leptin
MAPK 1/3
Vital pulp therapy
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Summary:•Leptin stimulates DSPP expression via PI3K pathway in human dental pulp.•Leptin stimulates DMP-1 synthesis via MAPK pathway in human dental pulp.•DSSP and DMP-1 are non-collagenous proteins implicated in dentinogenesis.•These results support the role of leptin in pulpal reparative response.•The use of leptin in vital pulp therapy could enhance pulp reparative response. To investigate the physiological function of leptin in human dental pulp, and to determine the specific pathways implicated in its effect. Twenty-seven dental pulp samples were obtained from human third molars. Pulp samples were treated with or without human recombinant leptin. Leptin functional effect was analyzed in terms of regulation of the synthesis levels of DSPP and DMP-1, determined by immunoblot. Leptin stimulated DMP-1 and DSPP synthesis in all human dental pulp specimens. The stimulatory effect of leptin on DMP-1 and DSPP synthesis was partially prevented by blocking mitogen-activated protein kinase (MAPK 1/3) and phosphatidylinositol 3 kinase (PI3K) pathways, respectively. The present study demonstrates the functional effect of leptin in human dental pulp stimulating the expression of DMP-1 and DSPP, both proteins implicated in dentinogenesis. Leptin stimulates DSPP expression via PI3K pathway and DMP-1 synthesis via MAPK 1/3 pathway. These results support the role of leptin in pulpal reparative response, opening a new research line that could have translational application to the clinic in vital pulp therapy procedures.
ISSN:0003-9969
1879-1506
DOI:10.1016/j.archoralbio.2018.11.019