Comparison of the glycosaminoglycans isolated from the skin and head cartilage of Gould's arrow squid ( Nototodarus gouldi)

The glycosaminoglycans from the skin and head cartilage of the squid Nototodarus gouldi have been isolated and characterised by constituent disaccharide and neutral sugar analysis, 13C nuclear magnetic resonance (NMR) spectroscopy, anion exchange and size exclusion chromatography. The glycosaminogly...

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Bibliographic Details
Published in:Carbohydrate polymers 2000-04, Vol.41 (4), p.357-364
Main Authors: Falshaw, R., Hubl, U., Ofman, D., Slim, G.C., Amjad Tariq, M., Watt, D.K., Yorke, S.C.
Format: Article
Language:English
Subjects:
Applied sciences
Biochemistry. Physiology. Immunology
Biological and medical sciences
Cartilage
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Glycosaminoglycans
Gould's arrow squid
Invertebrates
Molecular weight
Mollusca
Natural polymers
Nototodarus gouldi
Nuclear magnetic resonance spectroscopy
Physicochemistry of polymers
Physiology. Development
Size exclusion chromatography
Skin
Starch and polysaccharides
Sugars
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Summary:The glycosaminoglycans from the skin and head cartilage of the squid Nototodarus gouldi have been isolated and characterised by constituent disaccharide and neutral sugar analysis, 13C nuclear magnetic resonance (NMR) spectroscopy, anion exchange and size exclusion chromatography. The glycosaminoglycans from both tissues are chondroitin sulphate species. The skin consists principally of unsulphated but relatively highly glycosylated material. The chondroitin sulphate from the head cartilage is more highly sulphated, predominantly C-4,6diS (chondroitin sulphate E), with a higher molecular weight than the skin derived material but somewhat less highly glycosylated. To provide a standard for the assignment of the 13C NMR spectrum, C-4,6diS was chemically prepared from bovine tracheal chondroitin sulphate. This showed that it is not possible to distinguish between a mixture of the monosulphates, C-4S and C-6S, and the C-4,6diS by one-dimensional and simple two-dimensional 13C NMR techniques.
ISSN:0144-8617
1879-1344
DOI:10.1016/S0144-8617(99)00103-4