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Comparison of the glycosaminoglycans isolated from the skin and head cartilage of Gould's arrow squid ( Nototodarus gouldi)
The glycosaminoglycans from the skin and head cartilage of the squid Nototodarus gouldi have been isolated and characterised by constituent disaccharide and neutral sugar analysis, 13C nuclear magnetic resonance (NMR) spectroscopy, anion exchange and size exclusion chromatography. The glycosaminogly...
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| Published in: | Carbohydrate polymers 2000-04, Vol.41 (4), p.357-364 |
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| Main Authors: | , , , , , , |
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| cites | cdi_FETCH-LOGICAL-c433t-5edfdb5a7a3d4906ddf39f7c5f62531df080f64ca87a6b8b2b780d362c8a0efc3 |
| container_end_page | 364 |
| container_issue | 4 |
| container_start_page | 357 |
| container_title | Carbohydrate polymers |
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| creator | Falshaw, R. Hubl, U. Ofman, D. Slim, G.C. Amjad Tariq, M. Watt, D.K. Yorke, S.C. |
| description | The glycosaminoglycans from the skin and head cartilage of the squid
Nototodarus gouldi have been isolated and characterised by constituent disaccharide and neutral sugar analysis,
13C nuclear magnetic resonance (NMR) spectroscopy, anion exchange and size exclusion chromatography. The glycosaminoglycans from both tissues are chondroitin sulphate species. The skin consists principally of unsulphated but relatively highly glycosylated material. The chondroitin sulphate from the head cartilage is more highly sulphated, predominantly C-4,6diS (chondroitin sulphate E), with a higher molecular weight than the skin derived material but somewhat less highly glycosylated. To provide a standard for the assignment of the
13C NMR spectrum, C-4,6diS was chemically prepared from bovine tracheal chondroitin sulphate. This showed that it is not possible to distinguish between a mixture of the monosulphates, C-4S and C-6S, and the C-4,6diS by one-dimensional and simple two-dimensional
13C NMR techniques. |
| doi_str_mv | 10.1016/S0144-8617(99)00103-4 |
| format | article |
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Nototodarus gouldi have been isolated and characterised by constituent disaccharide and neutral sugar analysis,
13C nuclear magnetic resonance (NMR) spectroscopy, anion exchange and size exclusion chromatography. The glycosaminoglycans from both tissues are chondroitin sulphate species. The skin consists principally of unsulphated but relatively highly glycosylated material. The chondroitin sulphate from the head cartilage is more highly sulphated, predominantly C-4,6diS (chondroitin sulphate E), with a higher molecular weight than the skin derived material but somewhat less highly glycosylated. To provide a standard for the assignment of the
13C NMR spectrum, C-4,6diS was chemically prepared from bovine tracheal chondroitin sulphate. This showed that it is not possible to distinguish between a mixture of the monosulphates, C-4S and C-6S, and the C-4,6diS by one-dimensional and simple two-dimensional
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Nototodarus gouldi have been isolated and characterised by constituent disaccharide and neutral sugar analysis,
13C nuclear magnetic resonance (NMR) spectroscopy, anion exchange and size exclusion chromatography. The glycosaminoglycans from both tissues are chondroitin sulphate species. The skin consists principally of unsulphated but relatively highly glycosylated material. The chondroitin sulphate from the head cartilage is more highly sulphated, predominantly C-4,6diS (chondroitin sulphate E), with a higher molecular weight than the skin derived material but somewhat less highly glycosylated. To provide a standard for the assignment of the
13C NMR spectrum, C-4,6diS was chemically prepared from bovine tracheal chondroitin sulphate. This showed that it is not possible to distinguish between a mixture of the monosulphates, C-4S and C-6S, and the C-4,6diS by one-dimensional and simple two-dimensional
13C NMR techniques.</description><subject>Applied sciences</subject><subject>Biochemistry. Physiology. Immunology</subject><subject>Biological and medical sciences</subject><subject>Cartilage</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycosaminoglycans</subject><subject>Gould's arrow squid</subject><subject>Invertebrates</subject><subject>Molecular weight</subject><subject>Mollusca</subject><subject>Natural polymers</subject><subject>Nototodarus gouldi</subject><subject>Nuclear magnetic resonance spectroscopy</subject><subject>Physicochemistry of polymers</subject><subject>Physiology. Development</subject><subject>Size exclusion chromatography</subject><subject>Skin</subject><subject>Starch and polysaccharides</subject><subject>Sugars</subject><issn>0144-8617</issn><issn>1879-1344</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNqFkE1P3DAQhi3USmxpf0IlH6oCh4AdO45zQmhVoBKCA-3ZmvXH4jaJF09ShPjzJLsIjrUPHsnPvKN5CPnK2QlnXJ3eMS5loRWvj5rmmDHORCH3yILruim4kPIDWbwh--QT4h82HcXZgjwvU7eBHDH1NAU63Hu6bp9sQuhin-YSeqTTdwuDdzTk1G0h_Bt7Cr2j9x4ctZCH2MLazxmXaWzdIVLIOT1SfBijo0f0Jg3TdZBHpOuZiMefyccALfovr-8B-X3x49fyqri-vfy5PL8urBRiKCrvgltVUINwsmHKuSCaUNsqqLIS3AWmWVDSgq5BrfSqXNWaOaFKq4H5YMUB-b7L3eT0MHocTBfR-raF3qcRTcmFrrmSE1jtQJsTYvbBbHLsID8Zzsys2mxVm9mjaRqzVW3mvm-vAwAttCFDbyO-N5dcT7on7GyH-WnZf9Fngzb63noXs7eDcSn-Z9ALHjeU9Q</recordid><startdate>20000401</startdate><enddate>20000401</enddate><creator>Falshaw, R.</creator><creator>Hubl, U.</creator><creator>Ofman, D.</creator><creator>Slim, G.C.</creator><creator>Amjad Tariq, M.</creator><creator>Watt, D.K.</creator><creator>Yorke, S.C.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20000401</creationdate><title>Comparison of the glycosaminoglycans isolated from the skin and head cartilage of Gould's arrow squid ( Nototodarus gouldi)</title><author>Falshaw, R. ; Hubl, U. ; Ofman, D. ; Slim, G.C. ; Amjad Tariq, M. ; Watt, D.K. ; Yorke, S.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c433t-5edfdb5a7a3d4906ddf39f7c5f62531df080f64ca87a6b8b2b780d362c8a0efc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Applied sciences</topic><topic>Biochemistry. Physiology. Immunology</topic><topic>Biological and medical sciences</topic><topic>Cartilage</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycosaminoglycans</topic><topic>Gould's arrow squid</topic><topic>Invertebrates</topic><topic>Molecular weight</topic><topic>Mollusca</topic><topic>Natural polymers</topic><topic>Nototodarus gouldi</topic><topic>Nuclear magnetic resonance spectroscopy</topic><topic>Physicochemistry of polymers</topic><topic>Physiology. Development</topic><topic>Size exclusion chromatography</topic><topic>Skin</topic><topic>Starch and polysaccharides</topic><topic>Sugars</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Falshaw, R.</creatorcontrib><creatorcontrib>Hubl, U.</creatorcontrib><creatorcontrib>Ofman, D.</creatorcontrib><creatorcontrib>Slim, G.C.</creatorcontrib><creatorcontrib>Amjad Tariq, M.</creatorcontrib><creatorcontrib>Watt, D.K.</creatorcontrib><creatorcontrib>Yorke, S.C.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Carbohydrate polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Falshaw, R.</au><au>Hubl, U.</au><au>Ofman, D.</au><au>Slim, G.C.</au><au>Amjad Tariq, M.</au><au>Watt, D.K.</au><au>Yorke, S.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of the glycosaminoglycans isolated from the skin and head cartilage of Gould's arrow squid ( Nototodarus gouldi)</atitle><jtitle>Carbohydrate polymers</jtitle><date>2000-04-01</date><risdate>2000</risdate><volume>41</volume><issue>4</issue><spage>357</spage><epage>364</epage><pages>357-364</pages><issn>0144-8617</issn><eissn>1879-1344</eissn><coden>CAPOD8</coden><abstract>The glycosaminoglycans from the skin and head cartilage of the squid
Nototodarus gouldi have been isolated and characterised by constituent disaccharide and neutral sugar analysis,
13C nuclear magnetic resonance (NMR) spectroscopy, anion exchange and size exclusion chromatography. The glycosaminoglycans from both tissues are chondroitin sulphate species. The skin consists principally of unsulphated but relatively highly glycosylated material. The chondroitin sulphate from the head cartilage is more highly sulphated, predominantly C-4,6diS (chondroitin sulphate E), with a higher molecular weight than the skin derived material but somewhat less highly glycosylated. To provide a standard for the assignment of the
13C NMR spectrum, C-4,6diS was chemically prepared from bovine tracheal chondroitin sulphate. This showed that it is not possible to distinguish between a mixture of the monosulphates, C-4S and C-6S, and the C-4,6diS by one-dimensional and simple two-dimensional
13C NMR techniques.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><doi>10.1016/S0144-8617(99)00103-4</doi><tpages>8</tpages></addata></record> |
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| ispartof | Carbohydrate polymers, 2000-04, Vol.41 (4), p.357-364 |
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| subjects | Applied sciences Biochemistry. Physiology. Immunology Biological and medical sciences Cartilage Exact sciences and technology Fundamental and applied biological sciences. Psychology Glycosaminoglycans Gould's arrow squid Invertebrates Molecular weight Mollusca Natural polymers Nototodarus gouldi Nuclear magnetic resonance spectroscopy Physicochemistry of polymers Physiology. Development Size exclusion chromatography Skin Starch and polysaccharides Sugars |
| title | Comparison of the glycosaminoglycans isolated from the skin and head cartilage of Gould's arrow squid ( Nototodarus gouldi) |
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