Effect of oxygen on the growth of Clostridium butyricum (type species of the genus Clostridium), and the distribution of enzymes for oxygen and for active oxygen species in Clostridia

Clostridia are well-known obligatory anaerobic bacteria which cannot utilize oxygen, or otherwise die in oxygenated environments. Clostridium butyricum, the type species of the genus Clostridium, possesses the ability to consume oxygen in amounts proportional to the size of the inoculum. Oxygen cons...

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Bibliographic Details
Published in:Journal of fermentation and bioengineering 1998-01, Vol.86 (4), p.368-372
Main Authors: Kawasaki, Sinji, Nakagawa, Tomoyuki, Nishiyama, Yoshitaka, Benno, Yoshimi, Uchimura, Tai, Komagata, Kazuo, Kozaki, Michio, Niimura, Youichi
Format: Article
Language:English
Subjects:
AhpC
Bacteriology
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
CLOSTRIDIUM BUTYRICUM
CRECIMIENTO
CROISSANCE
Dissolution
Enzymes
Fundamental and applied biological sciences. Psychology
GROWTH
Growth kinetics
Metabolism. Enzymes
Microbiology
Mission oriented research
NADH oxidase
NADPH oxidase
Oxidation
oxidative damage
OXIDOREDUCTASES
OXIDORREDUCTASAS
OXIGENO
OXYDOREDUCTASE
OXYGEN
oxygen tolerance
OXYGENE
Physiology and metabolism
SOD
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Summary:Clostridia are well-known obligatory anaerobic bacteria which cannot utilize oxygen, or otherwise die in oxygenated environments. Clostridium butyricum, the type species of the genus Clostridium, possesses the ability to consume oxygen in amounts proportional to the size of the inoculum. Oxygen consumption was observed when NADH and NADPH were added to the cell extract of this strain. NADH oxidase and NADPH oxidase activities were also detected in all of the tested strains of the genus Clostridium. C. butyricum ceased growing while consuming oxygen in the medium. After consumption of all the dissolved oxygen, C. butyricum resumed growth at a rate equivalent to its anaerobic growth rate, suggesting that no oxidative damage based on oxygen reduction occurs in vivo. As scavengers for active oxygen species, the activities of NADH NADPH peroxidase and SOD were detected in C. butyricum. Furthermore, the activities of these enzymes are distributed widely in the genus Clostridium.
ISSN:0922-338X
DOI:10.1016/S0922-338X(99)89006-0