Scanning Force Microscopy Images of Cytochrome c Oxidase Immobilized in an Electrode-Supported Lipid Bilayer Membrane

Scanning force microscopic images of cytochrome c oxidase immobilized within an electrode-supported lipid bilayer membrane are reported. These images represent the first direct evidence that the microscopic architecture of this important model system effectively mimics the microstructure proposed fo...

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Bibliographic Details
Published in:Langmuir 1998-11, Vol.14 (23), p.6628-6631
Main Authors: Burgess, James D, Jones, Vivian W, Porter, Marc D, Rhoten, Melissa C, Hawkridge, Fred M
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Biological membranes
Electrodes
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Lipids
Microscopic examination
Molecular structure
Monomers
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Summary:Scanning force microscopic images of cytochrome c oxidase immobilized within an electrode-supported lipid bilayer membrane are reported. These images represent the first direct evidence that the microscopic architecture of this important model system effectively mimics the microstructure proposed for the inner mitochondrial membrane. The images reveal (1) that the oxidase is present as monomers and small aggregates within the supported membrane and (2) that the oxidase constitutes ∼20% of the lipid bilayer membrane. The latter finding allows an estimation of the lower limit for the minimum turnover rate (∼0.5 electrons/s) required for the transition of the oxidase from its resting to pulsed kinetic state.
ISSN:0743-7463
1520-5827
DOI:10.1021/la980648d