Immobilization of polyphenol oxidase on nylon and polyether-sulphone membranes: Effect on product formation

Preliminary studies were made of the potential use of a membrane-immobilized enzymatic method for the treatment of p-cresol-containing wastewater. The enzyme polyphenol oxidase was immobilized onto hydrophobic polyethersulphone capillary membranes and hydrophilic nylon flat-sheet membranes, by adsor...

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Bibliographic Details
Published in:Desalination 1998-08, Vol.115 (3), p.307-312
Main Authors: Boshoff, A, Edwards, W, Leukes, W D, Rose, P D, Burton, S G
Format: Article
Language:English
Subjects:
Adsorption
Aldehydes
Crosslinking
Enzyme immobilization
Ketones
Nylon polymers
Polyethers
Polymeric membranes
Polysulfones
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Summary:Preliminary studies were made of the potential use of a membrane-immobilized enzymatic method for the treatment of p-cresol-containing wastewater. The enzyme polyphenol oxidase was immobilized onto hydrophobic polyethersulphone capillary membranes and hydrophilic nylon flat-sheet membranes, by adsorption, and by adsorption with glutaraldehyde cross-linking, respectively. It was found that the intermediate product of the polyphenol oxidase reaction, 4-methylcatechol, was detected when the enzyme was immobilized on the nylon membranes or was not immobilized, but only the o-quinone final product was detected when polyethersulphone was used as the immobilization matrix.
ISSN:0011-9164