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The presence of osteocalcin, osteopontin and reactive oxygen species‐positive cells in pulp tissue after dental bleaching
Aim To analyse the influence of H2O2 on pulp repair through osteocalcin and osteopontin immunolabelling and in cellular defence by using the antireactive oxygen species (ROS) antibody. Methodology The maxillary molars of 50 rats were treated with 35% H2O2 (Ble groups) or placebo gel (control groups)...
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| Published in: | International endodontic journal 2019-05, Vol.52 (5), p.665-675 |
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| Main Authors: | , , , , , , , |
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| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c3539-e67e9187fb90331180949518e748cf3579a825057308648129573e736500d9373 |
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| cites | cdi_FETCH-LOGICAL-c3539-e67e9187fb90331180949518e748cf3579a825057308648129573e736500d9373 |
| container_end_page | 675 |
| container_issue | 5 |
| container_start_page | 665 |
| container_title | International endodontic journal |
| container_volume | 52 |
| creator | Benetti, F. Briso, A. L. F. Carminatti, M. Araújo Lopes, J. M. Barbosa, J. G. Ervolino, E. Gomes‐Filho, J. E. Cintra, L. T. A. |
| description | Aim
To analyse the influence of H2O2 on pulp repair through osteocalcin and osteopontin immunolabelling and in cellular defence by using the antireactive oxygen species (ROS) antibody.
Methodology
The maxillary molars of 50 rats were treated with 35% H2O2 (Ble groups) or placebo gel (control groups). At 0 h and 2, 7, 15 and 30 days (n = 10 hemimaxillae), the rats were killed and pulp tissue was evaluated using inflammation and immunolabelling scores (osteocalcin/osteopontin); ROS‐positive cells were counted. Paired t‐test and Wilcoxon signed‐rank test were used (P 0.05). Osteocalcin was absent in the Ble at 0 h, moderate at 2 days and increased thereafter, differing from the controls at all two periods (P |
| doi_str_mv | 10.1111/iej.13049 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2139568650</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2139568650</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3539-e67e9187fb90331180949518e748cf3579a825057308648129573e736500d9373</originalsourceid><addsrcrecordid>eNp10cFOHCEYB3DSaLpb24Mv0JB4sYmjMMAMHM1m22o28aJnwrLfKJtZmMKMuumlj9Bn7JOUddSDiVyA8Ms_X_gjdEjJKc3rzMH6lDLC1Qc0pawSRSkU3UNTQjkrSinFBH1KaU0IEYTRj2iSrZS8ElP0-_oOcBchgbeAQ4ND6iFY01rnT8ZLF3zvPDZ-hSMY27v7DB-3t-Bx6sA6SP_-_O1Cck8vFto24ey7oe1w71IaAJumh4hX4HvT4mWbU-6cv_2M9hvTJvjyvB-gm-_z69nPYnH142J2vigsE0wVUNWgqKybpSKMUSqJ4kpQCTWXtmGiVkaWgoiaEVlxSUuVj1DnfyBkpVjNDtDxmNvF8GuA1OuNS7s5jYcwJF1SpkQls8_06A1dhyH6PJ0uSyIU51zu1LdR2RhSitDoLrqNiVtNid41onMj-qmRbL8-Jw7LDaxe5UsFGZyN4MG1sH0_SV_ML8fI_6nKlLg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2205944480</pqid></control><display><type>article</type><title>The presence of osteocalcin, osteopontin and reactive oxygen species‐positive cells in pulp tissue after dental bleaching</title><source>Wiley-Blackwell Read & Publish Collection</source><creator>Benetti, F. ; Briso, A. L. F. ; Carminatti, M. ; Araújo Lopes, J. M. ; Barbosa, J. G. ; Ervolino, E. ; Gomes‐Filho, J. E. ; Cintra, L. T. A.</creator><creatorcontrib>Benetti, F. ; Briso, A. L. F. ; Carminatti, M. ; Araújo Lopes, J. M. ; Barbosa, J. G. ; Ervolino, E. ; Gomes‐Filho, J. E. ; Cintra, L. T. A.</creatorcontrib><description>Aim
To analyse the influence of H2O2 on pulp repair through osteocalcin and osteopontin immunolabelling and in cellular defence by using the antireactive oxygen species (ROS) antibody.
Methodology
The maxillary molars of 50 rats were treated with 35% H2O2 (Ble groups) or placebo gel (control groups). At 0 h and 2, 7, 15 and 30 days (n = 10 hemimaxillae), the rats were killed and pulp tissue was evaluated using inflammation and immunolabelling scores (osteocalcin/osteopontin); ROS‐positive cells were counted. Paired t‐test and Wilcoxon signed‐rank test were used (P < 0.05).
Results
The Ble group had necrosis in the coronal pulp at 0 h and in the occlusal third of the coronal pulp at 2 days; at 7, 15 and 30 days, no inflammation was noted similar to the controls (P > 0.05). Osteocalcin was absent in the Ble at 0 h, moderate at 2 days and increased thereafter, differing from the controls at all two periods (P < 0.05). Osteopontin was higher principally at 7 and 15 days in Ble groups, but differing with control groups from 2 days after bleaching (P < 0.05). The Ble group had more ROS‐positive cells in the pulp at 7 and 15 days (P < 0.05). Tertiary dentine was observed at 7 days, increasing thereafter (P < 0.05).
Conclusions
Post‐bleaching pulp repair was associated with increased osteocalcin over time. Osteopontin also participated in this process, and anti‐ROS was involved in cellular defence against H2O2.</description><identifier>ISSN: 0143-2885</identifier><identifier>EISSN: 1365-2591</identifier><identifier>DOI: 10.1111/iej.13049</identifier><identifier>PMID: 30488465</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Bleaching ; Dental pulp ; Dentistry ; Endodontics ; Hydrogen peroxide ; Molars ; Osteocalcin ; Osteopontin ; Reactive oxygen species ; Teeth ; tertiary dentine</subject><ispartof>International endodontic journal, 2019-05, Vol.52 (5), p.665-675</ispartof><rights>2018 International Endodontic Journal. Published by John Wiley & Sons Ltd</rights><rights>2018 International Endodontic Journal. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2019 International Endodontic Journal. Published by John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3539-e67e9187fb90331180949518e748cf3579a825057308648129573e736500d9373</citedby><cites>FETCH-LOGICAL-c3539-e67e9187fb90331180949518e748cf3579a825057308648129573e736500d9373</cites><orcidid>0000-0003-2348-7846 ; 0000-0001-5994-2287 ; 0000-0002-5459-353X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30488465$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Benetti, F.</creatorcontrib><creatorcontrib>Briso, A. L. F.</creatorcontrib><creatorcontrib>Carminatti, M.</creatorcontrib><creatorcontrib>Araújo Lopes, J. M.</creatorcontrib><creatorcontrib>Barbosa, J. G.</creatorcontrib><creatorcontrib>Ervolino, E.</creatorcontrib><creatorcontrib>Gomes‐Filho, J. E.</creatorcontrib><creatorcontrib>Cintra, L. T. A.</creatorcontrib><title>The presence of osteocalcin, osteopontin and reactive oxygen species‐positive cells in pulp tissue after dental bleaching</title><title>International endodontic journal</title><addtitle>Int Endod J</addtitle><description>Aim
To analyse the influence of H2O2 on pulp repair through osteocalcin and osteopontin immunolabelling and in cellular defence by using the antireactive oxygen species (ROS) antibody.
Methodology
The maxillary molars of 50 rats were treated with 35% H2O2 (Ble groups) or placebo gel (control groups). At 0 h and 2, 7, 15 and 30 days (n = 10 hemimaxillae), the rats were killed and pulp tissue was evaluated using inflammation and immunolabelling scores (osteocalcin/osteopontin); ROS‐positive cells were counted. Paired t‐test and Wilcoxon signed‐rank test were used (P < 0.05).
Results
The Ble group had necrosis in the coronal pulp at 0 h and in the occlusal third of the coronal pulp at 2 days; at 7, 15 and 30 days, no inflammation was noted similar to the controls (P > 0.05). Osteocalcin was absent in the Ble at 0 h, moderate at 2 days and increased thereafter, differing from the controls at all two periods (P < 0.05). Osteopontin was higher principally at 7 and 15 days in Ble groups, but differing with control groups from 2 days after bleaching (P < 0.05). The Ble group had more ROS‐positive cells in the pulp at 7 and 15 days (P < 0.05). Tertiary dentine was observed at 7 days, increasing thereafter (P < 0.05).
Conclusions
Post‐bleaching pulp repair was associated with increased osteocalcin over time. Osteopontin also participated in this process, and anti‐ROS was involved in cellular defence against H2O2.</description><subject>Bleaching</subject><subject>Dental pulp</subject><subject>Dentistry</subject><subject>Endodontics</subject><subject>Hydrogen peroxide</subject><subject>Molars</subject><subject>Osteocalcin</subject><subject>Osteopontin</subject><subject>Reactive oxygen species</subject><subject>Teeth</subject><subject>tertiary dentine</subject><issn>0143-2885</issn><issn>1365-2591</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp10cFOHCEYB3DSaLpb24Mv0JB4sYmjMMAMHM1m22o28aJnwrLfKJtZmMKMuumlj9Bn7JOUddSDiVyA8Ms_X_gjdEjJKc3rzMH6lDLC1Qc0pawSRSkU3UNTQjkrSinFBH1KaU0IEYTRj2iSrZS8ElP0-_oOcBchgbeAQ4ND6iFY01rnT8ZLF3zvPDZ-hSMY27v7DB-3t-Bx6sA6SP_-_O1Cck8vFto24ey7oe1w71IaAJumh4hX4HvT4mWbU-6cv_2M9hvTJvjyvB-gm-_z69nPYnH142J2vigsE0wVUNWgqKybpSKMUSqJ4kpQCTWXtmGiVkaWgoiaEVlxSUuVj1DnfyBkpVjNDtDxmNvF8GuA1OuNS7s5jYcwJF1SpkQls8_06A1dhyH6PJ0uSyIU51zu1LdR2RhSitDoLrqNiVtNid41onMj-qmRbL8-Jw7LDaxe5UsFGZyN4MG1sH0_SV_ML8fI_6nKlLg</recordid><startdate>201905</startdate><enddate>201905</enddate><creator>Benetti, F.</creator><creator>Briso, A. L. F.</creator><creator>Carminatti, M.</creator><creator>Araújo Lopes, J. M.</creator><creator>Barbosa, J. G.</creator><creator>Ervolino, E.</creator><creator>Gomes‐Filho, J. E.</creator><creator>Cintra, L. T. A.</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2348-7846</orcidid><orcidid>https://orcid.org/0000-0001-5994-2287</orcidid><orcidid>https://orcid.org/0000-0002-5459-353X</orcidid></search><sort><creationdate>201905</creationdate><title>The presence of osteocalcin, osteopontin and reactive oxygen species‐positive cells in pulp tissue after dental bleaching</title><author>Benetti, F. ; Briso, A. L. F. ; Carminatti, M. ; Araújo Lopes, J. M. ; Barbosa, J. G. ; Ervolino, E. ; Gomes‐Filho, J. E. ; Cintra, L. T. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3539-e67e9187fb90331180949518e748cf3579a825057308648129573e736500d9373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Bleaching</topic><topic>Dental pulp</topic><topic>Dentistry</topic><topic>Endodontics</topic><topic>Hydrogen peroxide</topic><topic>Molars</topic><topic>Osteocalcin</topic><topic>Osteopontin</topic><topic>Reactive oxygen species</topic><topic>Teeth</topic><topic>tertiary dentine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Benetti, F.</creatorcontrib><creatorcontrib>Briso, A. L. F.</creatorcontrib><creatorcontrib>Carminatti, M.</creatorcontrib><creatorcontrib>Araújo Lopes, J. M.</creatorcontrib><creatorcontrib>Barbosa, J. G.</creatorcontrib><creatorcontrib>Ervolino, E.</creatorcontrib><creatorcontrib>Gomes‐Filho, J. E.</creatorcontrib><creatorcontrib>Cintra, L. T. A.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>International endodontic journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Benetti, F.</au><au>Briso, A. L. F.</au><au>Carminatti, M.</au><au>Araújo Lopes, J. M.</au><au>Barbosa, J. G.</au><au>Ervolino, E.</au><au>Gomes‐Filho, J. E.</au><au>Cintra, L. T. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The presence of osteocalcin, osteopontin and reactive oxygen species‐positive cells in pulp tissue after dental bleaching</atitle><jtitle>International endodontic journal</jtitle><addtitle>Int Endod J</addtitle><date>2019-05</date><risdate>2019</risdate><volume>52</volume><issue>5</issue><spage>665</spage><epage>675</epage><pages>665-675</pages><issn>0143-2885</issn><eissn>1365-2591</eissn><abstract>Aim
To analyse the influence of H2O2 on pulp repair through osteocalcin and osteopontin immunolabelling and in cellular defence by using the antireactive oxygen species (ROS) antibody.
Methodology
The maxillary molars of 50 rats were treated with 35% H2O2 (Ble groups) or placebo gel (control groups). At 0 h and 2, 7, 15 and 30 days (n = 10 hemimaxillae), the rats were killed and pulp tissue was evaluated using inflammation and immunolabelling scores (osteocalcin/osteopontin); ROS‐positive cells were counted. Paired t‐test and Wilcoxon signed‐rank test were used (P < 0.05).
Results
The Ble group had necrosis in the coronal pulp at 0 h and in the occlusal third of the coronal pulp at 2 days; at 7, 15 and 30 days, no inflammation was noted similar to the controls (P > 0.05). Osteocalcin was absent in the Ble at 0 h, moderate at 2 days and increased thereafter, differing from the controls at all two periods (P < 0.05). Osteopontin was higher principally at 7 and 15 days in Ble groups, but differing with control groups from 2 days after bleaching (P < 0.05). The Ble group had more ROS‐positive cells in the pulp at 7 and 15 days (P < 0.05). Tertiary dentine was observed at 7 days, increasing thereafter (P < 0.05).
Conclusions
Post‐bleaching pulp repair was associated with increased osteocalcin over time. Osteopontin also participated in this process, and anti‐ROS was involved in cellular defence against H2O2.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>30488465</pmid><doi>10.1111/iej.13049</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-2348-7846</orcidid><orcidid>https://orcid.org/0000-0001-5994-2287</orcidid><orcidid>https://orcid.org/0000-0002-5459-353X</orcidid></addata></record> |
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| identifier | ISSN: 0143-2885 |
| ispartof | International endodontic journal, 2019-05, Vol.52 (5), p.665-675 |
| issn | 0143-2885 1365-2591 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2139568650 |
| source | Wiley-Blackwell Read & Publish Collection |
| subjects | Bleaching Dental pulp Dentistry Endodontics Hydrogen peroxide Molars Osteocalcin Osteopontin Reactive oxygen species Teeth tertiary dentine |
| title | The presence of osteocalcin, osteopontin and reactive oxygen species‐positive cells in pulp tissue after dental bleaching |
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