Heavy Metal Tolerance in Chromogenic and Non-Chromogenic Marine Bacteria from Arabian Gulf

A simple method - direct agar diffusion assay - was optimised for rapid assessment of heavy metal toxicity to marine chromogenic and non-chromogenic bacteria. The procedure involved spotting of a 10 microliter test solution on the seeded agar plate and incubation of the plates at 30°C to accelerate...

Full description

Saved in:
Bibliographic Details
Published in:Environmental monitoring and assessment 1999-12, Vol.59 (3), p.321-330
Main Author: Chandy, Joseph P
Format: Article
Language:English
Subjects:
Agar
Arabian Gulf
Assays
Bacteria
Environmental monitoring
Heavy metals
Metal concentrations
Persian Gulf
Toxicity
Toxicology
Water bacteriology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A simple method - direct agar diffusion assay - was optimised for rapid assessment of heavy metal toxicity to marine chromogenic and non-chromogenic bacteria. The procedure involved spotting of a 10 microliter test solution on the seeded agar plate and incubation of the plates at 30°C to accelerate bacterial growth. Under optimum conditions, test results were obtainable within 12-18 hr instead of 96 hr incubation time generally required for a marine bacterial assay by conventional agar plate methods. A range of sixteen heavy metals, each at 5 different concentrations was tested. Toxicity was demonstrated by the formation of a clear zone of growth inhibition around the point of application. Toxicity of tested chemicals could be easily demonstrated at concentrations as low as 0.1 μg per spot on the agar plate. A dose dependent relation between metal concentration (μg/spot) and the diameter of the clear zone on agar plate was observed, suggesting potential of this method as an easy and economical tool in quantitative toxicology studies.[PUBLICATION ABSTRACT]
ISSN:0167-6369
1573-2959
DOI:10.1023/A:1006173722510