in vitro study of the toxic effects of Stachybotrys chartarum metabolites on lung cells

During a study of indoor fungal colonisation, several isolates of Stachybotrys chartarum were recovered, and the effects of metabolites from four isolates on lung epithelial Type II cells and alveolar macrophages were studied in vitro. All the isolates showed toxic effects on both cell types, and th...

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Bibliographic Details
Published in:Alternatives to laboratory animals 2007-03, Vol.35 (1), p.47-52
Main Authors: Kovacikova, Z, Tatrai, E, Pieckova, E, Tulinska, J, Pivovarova, Z, Matausic-Pisl, M, Kuricova, M, Wsolova, L
Format: Article
Language:English
Subjects:
Acid Phosphatase - metabolism
Alkaline Phosphatase - metabolism
Animals
Biological and medical sciences
Cells, Cultured
Dose-Response Relationship, Drug
Epithelial Cells - drug effects
Epithelial Cells - metabolism
in vitro studies
lungs
Macrophages, Alveolar - drug effects
Macrophages, Alveolar - metabolism
Medical sciences
Mycotoxins - isolation & purification
Mycotoxins - toxicity
Plant Lectins - metabolism
Pulmonary Alveoli - drug effects
Pulmonary Alveoli - metabolism
Rabbits
Rats
Stachybotrys
Stachybotrys - chemistry
Stachybotrys chartarum
toxicity testing
Toxicology
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Summary:During a study of indoor fungal colonisation, several isolates of Stachybotrys chartarum were recovered, and the effects of metabolites from four isolates on lung epithelial Type II cells and alveolar macrophages were studied in vitro. All the isolates showed toxic effects on both cell types, and they differed only in the extent of the changes induced. In Type II cells, the number of alkaline phosphatase positive cells was reduced, the pattern of Maclura pomifera agglutinin (MPA) binding was changed, and acid phosphatase activity in alveolar macrophages was diminished. In both cell types, the production of monocyte chemotactic protein-1 (MCP-1) and tumour necrosis factor-alpha (TNF-alpha) was changed, and parameters related to antioxidant status (superoxide dismutase, glutathione peroxidase, glutathione) were decreased.
ISSN:0261-1929
2632-3559
DOI:10.1177/026119290703500115