Novel introduction system for hydride generation-inductively coupled plasma mass spectrometry: Determination of selenium in biological materials

A novel, robust hydride generation system compatible with sample introduction with inductively coupled plasma mass spectrometry is described and applied to the determination of Se in biological tissues. A short reaction time (60 ms) and a rapid separation of the reaction products is obtained by mixi...

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Bibliographic Details
Published in:Journal of analytical atomic spectrometry 2000-02, Vol.15 (2), p.143-149
Main Authors: Moor, Christoph, Lam, Joseph WH, Sturgeon, Ralph E
Format: Article
Language:English
Subjects:
Hydrides
Plasma diagnostics
Selenium
Separation
Sodium compounds
Tissue
Online Access:Get full text
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Summary:A novel, robust hydride generation system compatible with sample introduction with inductively coupled plasma mass spectrometry is described and applied to the determination of Se in biological tissues. A short reaction time (60 ms) and a rapid separation of the reaction products is obtained by mixing the acidified sample and the sodium borohydride reductant solution at the tip of a cross-flow nebulizer. A modified Scott spray chamber serves as a gas-liquid separator, providing 30 s wash-in and wash-out times. Analytical results generated by external calibration and isotope dilution methodologies agree well with the certified values for Se in certified biological reference materials DORM-2 and DOLT-2. Detection limits for Se and other hydride forming elements, i.e., As, Sn and Sb, are below 10 ng l super(-1) with typical precision of 2% RSD at the 10 ng ml super(-1) level. Volatile species of Cu, Rh, Pd, Ag, In, Au, Hg, Tl and Pb are also produced, and estimated sensitivities and detection limits for these elements are reported.
ISSN:0267-9477
DOI:10.1039/a909296j