Fluorescence Excitation Emission Matrices of Human Tissue: A System for in vivo Measurement and Method of Data Analysis

We describe a system capable of measuring spatially resolved reflectance spectra from 380 to 950 nm and fluorescence excitation emission matrices from 330 to 500 nm excitation and 380 to 700 nm emission in vivo. System performance was compared to that of a standard scanning spectrofluorimeter. This...

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Bibliographic Details
Published in:Applied spectroscopy 1999-03, Vol.53 (3), p.302-311
Main Authors: Zuluaga, Andrés F., Utzinger, Urs, Durkin, Anthony, Fuchs, Holger, Gillenwater, Ann, Jacob, Rhonda, Kemp, Bonnie, Fan, James, Richards-Kortum, Rebecca
Format: Article
Language:English
Subjects:
Correlation methods
Diagnosis
Fiber optic sensors
Fluorescence
Probes
Scattering
Spectrum analysis
Tissue
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Summary:We describe a system capable of measuring spatially resolved reflectance spectra from 380 to 950 nm and fluorescence excitation emission matrices from 330 to 500 nm excitation and 380 to 700 nm emission in vivo. System performance was compared to that of a standard scanning spectrofluorimeter. This “FastEEM” system was used to interrogate human normal and neoplastic oral cavity mucosa in vivo. Measurements were made through a fiber-optic probe and require 4 min total measurement time. We present a method based on autocorrelation vectors to identify excitation and emission wavelengths where the spectra of normal and pathologic tissues differ most. The FastEEM system provides a tool with which to study the relative diagnostic ability of changes in absorption, scattering, and fluorescence properties of tissue.
ISSN:0003-7028
1943-3530
DOI:10.1366/0003702991946695