effects of multiple metal contamination on ectomycorrhizal Scots pine (Pinus sylvestris) seedlings

• Published in: Environmental pollution (1987) 1999-09, Vol.106 (3), p.413-424
• Main Authors: Hartley, J, Cairney, J.W.G, Freestone, P, Woods, C, Meharg, A.A
• Format: Article
• Language: English
• Subjects: Animal, plant and microbial ecology; Antimony; Applied ecology; Biological and medical sciences; Cadmium; Contamination; Copper; Ecotoxicology, biological effects of pollution; ectomycorrhizae; Effects of pollution and side effects of pesticides on plants and fungi; Fundamental and applied biological sciences. Psychology; Fungi; heavy metals; Lead; phytotoxicity; Pinus sylvestris; Pollution detection; soil pollution; Toxicity; Zinc
• ISSN: 0269-7491; 1873-6424
• DOI: 10.1016/s0269-7491(99)00095-0

Experiments were conducted to investigate the effects of single and multiple metal contamination (Cd, Pb, Zn, Sb, Cu) on Scots pine seedlings colonised by ectomycorrhizal (ECM) fungi from natural soil inoculum. Seedlings were grown in either contaminated field soil from the site of a chemical accident, soils amended with five metals contaminating the site, or in soil from an uncontaminated control site. Although contaminated and metal-amended soil significantly inhibited root and shoot growth of the Scots pine seedlings, total root tip density was not affected. Of the five metals tested in amended soils, Cd was the most toxic to ECM Scots pine. Field-contaminated soil had a toxic effect on ECM fungi associated with Scots pine seedlings and caused shifts in ECM species composition on ECM seedlings. When compared to soils amended with only one metal, soils amended with a combination of all five metals tested had lower relative toxicity and less accumulation of Pb, Zn and Sb into seedlings. This would indicate that the toxicity of multiple metal contamination cannot be predicted from the individual toxicity of the metals investigated.