Systemic anti-IFN-γ treatment and role of macrophage subsets in the foreign body reaction to dermal sheep collagen in rats

• Published in: Journal of biomedical materials research 2000-03, Vol.49 (3), p.297-304
• Main Authors: Khouw, I. M. S. L., van Wachem, P. B., van der Worp, R. J., van den Berg, T. K., de Leij, L. F. M. H., van Luyn, M. J. A.
• Format: Article
• Language: English
• Subjects: Animals; Antibodies - administration & dosage; Antigen-antibody reactions; Biocompatibility; Biocompatible Materials - toxicity; Biodegradation; Biological and medical sciences; Cells; Collagen; Collagen - immunology; Collagen - toxicity; foreign body reaction; Foreign-Body Reaction - immunology; Foreign-Body Reaction - pathology; Foreign-Body Reaction - prevention & control; Impregnation; Interferon-gamma - antagonists & inhibitors; interferon-γ; Interferons; Macrophage Activation; macrophages; Macrophages - classification; Macrophages - immunology; Macrophages - pathology; major histocompatibility complex class II; Male; Medical sciences; Rats; Sheep; Skin - immunology; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments
• ISSN: 0021-9304; 1097-4636
• DOI: 10.1002/(SICI)1097-4636(20000305)49:3<297::AID-JBM1>3.0.CO;2-0

The application of a biomaterial induces a foreign body reaction. By controlling this reaction, biocompatibility could be improved. We previously demonstrated that impregnation of a biodegradable biomaterial with antibodies against interferon‐γ (IFN‐γ) inhibits the foreign body reaction. In this study we investigate whether systemic administration of the antibody can induce similar reactions. Several parameters are compared between control and anti‐IFN‐γ‐treated rats: cellular ingrowth; degradation of the biomaterial; ingrowth of macrophage (MØ) subsets, T cells, B cells, NK cells, and granulocytes; and expression of the major histocompatibility complex class II (MHC class II) molecule on antigen presenting cells. Treatment with anti‐IFN‐γ results in increased cellular ingrowth and biomaterial degradation and a decreased expression of MHC class II. Overall, systemic treatment with anti‐IFN‐γ is insufficient to modulate the foreign body reaction. This suggests an alternative mechanism for MØ activation besides IFN‐γ. The role of T cells and MØ subsets in the foreign body reaction is discussed. © 2000 John Wiley & Sons, Inc. J Biomed Mater Res, 49, 297–304, 2000.