The quantification of proteolytic enzyme bioaerosols using flow injection analysis with fluorescence detection

The detection and quantification of proteolytic enzymes after capture in the form of a bioaerosol was investigated. Flow injection analysis with fluorescence detection was used for the process of enzyme capture and was in turn facilitated by the use of Texas Red succinimidyl ester. Enzyme concentrat...

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Bibliographic Details
Published in:Journal of aerosol science 2000-09, Vol.31 (SUPPL. 1), p.741-742
Main Authors: Grieveson, L.A., Sykes, D., Cumming, R.H., Rowell, F.J.
Format: Article
Language:English
Subjects:
Analysis methods
Applied sciences
Atmospheric pollution
Bioaerosols
Bioreactors
Cellulose
Enzymes
Esters
Exact sciences and technology
Fluorescence
gelatin
Gels
Pollution
proteolytic enzymes
texas red succinimidyl ester
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Summary:The detection and quantification of proteolytic enzymes after capture in the form of a bioaerosol was investigated. Flow injection analysis with fluorescence detection was used for the process of enzyme capture and was in turn facilitated by the use of Texas Red succinimidyl ester. Enzyme concentration was calibrated constantly by this method.
ISSN:0021-8502
1879-1964
DOI:10.1016/S0021-8502(00)90751-1