Isolation of a novel microorganism, Pestalotia heterocornis, producing paclitaxel

Pestalotia heterocornis was isolated from soil collected in yew forest and was shown to produce paclitaxel in semisynthetic liquid media. The presence of paclitaxel in the fungal extract was confirmed by FAB mass spectrometry and NMR spectroscopy. The maximum yield of paclitaxel was 31 microgram per...

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Bibliographic Details
Published in:Biotechnology and bioengineering 1999-09, Vol.64 (5), p.620-623
Main Authors: Noh, M.J, Yang, J.G, Kim, K.S, Yoon, Y.M, Kang, K.A, Han, H.Y, Shim, S.B, Park, H.J
Format: Article
Language:English
Subjects:
Biological and medical sciences
biological production
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Extraction
Fermentation
Fundamental and applied biological sciences. Psychology
fungi
Isolation and description
Mass spectrometry
Microbiology
Microorganisms
Mission oriented research
Nuclear magnetic resonance spectroscopy
paclitaxel
Pestalotia heterocornis
Soils
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Summary:Pestalotia heterocornis was isolated from soil collected in yew forest and was shown to produce paclitaxel in semisynthetic liquid media. The presence of paclitaxel in the fungal extract was confirmed by FAB mass spectrometry and NMR spectroscopy. The maximum yield of paclitaxel was 31 microgram per liter. Optimal paclitaxel production occurred after 5-7 days in a 20-liter scale fermentation at 23 degrees C. These results indicate that P. heterocornis is an excellent candidate for consideration in fermentation technology.
ISSN:0006-3592
1097-0290
DOI:10.1002/(SICI)1097-0290(19990905)64:5<620::AID-BIT13>3.0.CO;2-W