The effect of yeast elicitor on the growth and secondary metabolism of hairy root cultures of Salvia miltiorrhiza

Hairy root cultures of Salvia miltiorrhiza transformed with Agrobacterium rhizogenes ATCC 15834 produced a tiny amount of tanshinones and a constituent level of phenolic acids under normal growth conditions. Upon elicitation with yeast elicitor, the production of both phenolic acids and tanshinones...

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Bibliographic Details
Published in:Enzyme and microbial technology 2001-01, Vol.28 (1), p.100-105
Main Authors: Chen, Hui, Chena, Feng, Chiu, Francis C.K., Lo, Cindy M.Y.
Format: Article
Language:English
Subjects:
Agrobacterium rhizogenes
Biological and medical sciences
Biotechnology
Carboxylic acids
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Hairy roots
LC-MS
Liquid chromatography
lithospermic acid B
Mass spectrometry
Metabolism
Methods. Procedures. Technologies
Miscellaneous
Molecular weight
Phenolic acids
Plant cells and fungal cells
rosmarinic acid
Salvia miltiorrhiza
Tanshinones
Yeast
Yeast elicitor
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Summary:Hairy root cultures of Salvia miltiorrhiza transformed with Agrobacterium rhizogenes ATCC 15834 produced a tiny amount of tanshinones and a constituent level of phenolic acids under normal growth conditions. Upon elicitation with yeast elicitor, the production of both phenolic acids and tanshinones was enhanced. For example, the contents of two phenolic acids, rosmarinic acid and lithospermic acid B were elevated from 1.24% and 2.59% to 2.89% and 2.98% of dry wt, respectively while the intracellular content of cryptotanshinone increased from 0.001% to as much as 0.096% of dry wt. Yeast elicitor also improved the growth of hairy roots (from 3.9 g/l to 7.3 g/l on a dry wt basis). Liquid chromatography-mass spectrometry (LC-MS) was developed for simultaneous detection and identification of phenolic acids and tanshinones in the extracts of S. miltiorrhiza. Rosmarinic acid, lithospermic acid B, cryptotanshinone, tanshinone I, tanshinone IIA and tanshinone IIB were identified by comparison with standards available. Dihydrotanshinone I and methylenetanshiquinone were tentatively identified by the molecular weights and the elution comparable with the literature. An unknown compound with a molecular weight of 280 was found in yeast-elicitor treated hairy root cultures, which was one of the major tanshinones induced.
ISSN:0141-0229
1879-0909
DOI:10.1016/S0141-0229(00)00284-2