Yeast cell attachment: a tool modulating wall composition and resistance to 5-bromo-6-azauracil

The attachment of Candida utilis, Kluyveromyces lactis, and Saccharomyces cerevisiae cells stimulates an increase in the content of cell wall polysaccharides and mannoproteins, accompanied by increased resistance to the inhibitory effect of 5-bromo-6-azauracil. The covalent attachment of viable yeas...

Full description

Saved in:
Bibliographic Details
Published in:Enzyme and microbial technology 2000-06, Vol.26 (9-10), p.808-811
Main Authors: JIRKU, V, MASAK, J, CEJKOVA, A
Format: Article
Language:English
Subjects:
5-Bromo-6-azauracil
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Cell attachment
Cell culture
Cell immobilization
Covalent linkage
Fundamental and applied biological sciences. Psychology
Mission oriented research
Physiology and metabolism
Polysaccharides
Proteins
Wall polysaccharides
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The attachment of Candida utilis, Kluyveromyces lactis, and Saccharomyces cerevisiae cells stimulates an increase in the content of cell wall polysaccharides and mannoproteins, accompanied by increased resistance to the inhibitory effect of 5-bromo-6-azauracil. The covalent attachment of viable yeasts was accomplished (via dialdehyde-amino spacers) by reaction of aldehyde groups of the carrier with reactive amino groups in accessible cell surface proteins. The employed technique enables the optimization of yeast sources of β-1,3-, β-1,6- glucans, mannan, and mannoprotein. The modulatory effect of the cell attachment is discussed.
ISSN:0141-0229
1879-0909
DOI:10.1016/S0141-0229(00)00175-7