Mechanical loading influences ligament fibroblast behavior

To assess migration, artificially created cell-free areas in human ACL and MCL fibroblast monolayers were measured during periodic equi-biaxial strain application. In gene expression experiments, continuous cyclic equi-biaxial mechanical strains were applied to human ACL and MCL fibroblast monolayer...

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Bibliographic Details
Published in:Annals of biomedical engineering 2000-01, Vol.28 (SUPPL. 1), p.S-81
Main Authors: Hsieh, Adam, Ho, Jeffrey, Yang, Li, Sah, Robert, Akeson, Wayne, Sung, Kuo-Li
Format: Article
Language:English
Subjects:
Biomechanics
Bone
Cells
Collagen
Cytology
Genes
Ligaments
RNA
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Summary:To assess migration, artificially created cell-free areas in human ACL and MCL fibroblast monolayers were measured during periodic equi-biaxial strain application. In gene expression experiments, continuous cyclic equi-biaxial mechanical strains were applied to human ACL and MCL fibroblast monolayers and expression of types I and III collagens were determined. Also, a novel organ culture preparation for applying physiologic mechanical loads to intact bone-ACLbone complexes was developed and implemented to assess changes in type I collagen gene expression in rat ACLs. It was found that cell migration depended in a complex manner on both amplitude and frequency of periodic strains. Expression of types I and III collagens were differentially regulated between ACL and MCL fibroblasts.
ISSN:0090-6964