Kinetic observation of rapid electron transfer between pyrimidine electron adducts and sensitizers of riboflavin, flavin adenine dinucleotide (FAD) and chloranil: a pulse radiolysis study

Rapid electron transfer from electron adducts of pyrimidine nucleotides dCMP, TMP and UMP to sensitizers of riboflavin (RF), flavin adenine dinucleotide (FAD) and chloranil (CHL) were studied using the techniques of pulse radiolysis. Both spectroscopic and kinetic analyses showed that the transient...

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Bibliographic Details
Published in:Radiation physics and chemistry (Oxford, England : 1993) England : 1993), 2000-07, Vol.59 (1), p.61-66
Main Authors: Lu, Chang-Yuan, Lin, Wei-Zhen, Wang, Wen-Feng, Han, Zhen-Hui, Zheng, Zhi-De, Yao, Si-De
Format: Article
Language:English
Subjects:
Aromatic compounds
Charge transfer
Decomposition
DNA
Electron beams
Electron irradiation
Nitrogen compounds
Radiation damage
Reaction kinetics
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Summary:Rapid electron transfer from electron adducts of pyrimidine nucleotides dCMP, TMP and UMP to sensitizers of riboflavin (RF), flavin adenine dinucleotide (FAD) and chloranil (CHL) were studied using the techniques of pulse radiolysis. Both spectroscopic and kinetic analyses showed that the transient absorption spectra of pyrimidine electron adducts formed at first, and then were replaced by those of radical anions of RF, FAD and CHL after several microseconds of electron beam pulse, which indicated that electron transfer from electron adducts of pyrimidines to RF, FAD and CHL occurred. From buildup kinetics of sensitizer radical anions, a series of rate constants for electron transfer are determined respectively. RF and FAD can capture electron from pyrimidine electron adducts induced in irradiated cell, which would reduce possible repair of damaged targets due to the inhibition of charge recombination of radical ion pair. Thereby RF and FAD might play the role of radio-sensitizer in DNA damage process.
ISSN:0969-806X
1879-0895
DOI:10.1016/S0969-806X(00)00181-X