Immobilization of Nicotiana tabacum plant cell suspensions within calcium alginate gel beads for the production of enhanced amounts of scopolin

• Published in: Enzyme and microbial technology 2000-02, Vol.26 (2), p.229-234
• Main Authors: Gilleta, F, Roisin, C, Fliniaux, M.A, Jacquin–Dubreuil, A, Barbotin, J.N, Nava–Saucedo, J.E
• Format: Article
• Language: English
• Subjects: Alginate; Biological and medical sciences; Biotechnology; Calcium; Establishment of new cell lines, improvement of cultural methods, mass culture; Eukaryotic cell cultures; Excretion; Fundamental and applied biological sciences. Psychology; Gels; Growth kinetics; Immobilization; Methods. Procedures. Technologies; Morphology; Nicotiana tabacum; Plant cell culture; Plant cells and fungal cells; Polysaccharides; Scopolin; Secondary metabolites; Suspensions (fluids); Tobacco
• ISSN: 0141-0229; 1879-0909
• DOI: 10.1016/S0141-0229(99)00138-6

Scopolin-producing cells of Nicotiana tabacum were immobilized within Ca–alginate gel beads. Free cell suspensions accumulated scopolin within cytoplasmic compartments and cell disruption was necessary to recover scopolin. On the contrary, immobilized plant cells excreted considerable amounts of scopolin. Scopolin diffused throughout the gel matrix and reached the culture media. A large fraction of produced scopolin could then be recovered from the culture medium without disrupting cells. Immobilized N. tabacum cells produced more scopolin than free cell suspensions did (3.8 mg/g fresh weight biomass [into the culture media] versus 0.2 mg/g fresh weight biomass [intracellular]). Variation of the immobilization conditions revealed a marked influence on the behavior of N. tabacum plant cells: production of scopolin and enhanced excretion, cell growth, and morphological aspect of plant cell colonies. This excretion phenomenon could be used advantageously at an industrial production level.