Plasma protein adsorption onto cell attachment controlled ion implanted collagen

• Published in: Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms Beam interactions with materials and atoms, 2001-04, Vol.175, p.791-796
• Main Authors: Kurotobi, K, Kaibara, M, Suzuki, Y, Iwaki, M, Nakajima, H
• Format: Article
• Language: English
• Subjects: Adsorption; Anti-thrombogenicity; Artificial graft; Biomaterials; Cells; Collagen; Fibrinogen; Fibronectin; Grafts; Plasma applications
• ISSN: 0168-583X; 1872-9584
• DOI: 10.1016/S0168-583X(00)00526-7

Ion implantation into collagen (Type I) coated inner surfaces of test tubes with a length of 50 mm and inner diameter of 2 and 3 mm were performed to develop hybrid type small-diameter artificial vascular grafts. He + ion implanted collagen coated grafts with a fluence of 1×10 14 ions/ cm 2 replacing femoral arteries exhibited excellent graft patency. To obtain information about the relationship between plasma protein adsorption and antithrombogenicity of ion implanted collagen surfaces, protein adsorption measurements, platelet adhesion test, and animal study were performed. The amount of fibrinogen, fibronectin and albumin showed minimum value at a fluence of 1×10 14 ions/ cm 2 . The adsorption of fibrinogen and fibronectin to surfaces is known to promote the adhesion of platelets. The results indicated that antithrombogenicity of He + ion-implanted collagen with a fluence of 1×10 14 ions/ cm 2 was caused by the reduction of the amount of adsorbed proteins.