Heavy metal distribution in Suillus luteus mycorrhizas – as revealed by micro-PIXE analysis

Suillus luteus/Pinus sylvestris mycorrhizas, collected from zinc wastes in Southern Poland, were selected as potential biofilters on the basis of earlier studies carried out with energy dispersive spectrometry (EDS) microanalytical system coupled to scanning electron microscope (SEM) and transmissio...

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Published in:Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms Beam interactions with materials and atoms, 2001-07, Vol.181 (1), p.649-658
Main Authors: Turnau, K, Przybyłowicz, W.J, Mesjasz-Przybyłowicz, J
Format: Article
Language:English
Subjects:
Chemical fixation
Cryo-preparation
Ectomycorrhiza
Fungi
Heavy metal biofiltering
Heavy metals
PIXE
Scanning electron microscopy
Suillus luteus
Tissue
Transmission electron microscopy
Zinc
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Summary:Suillus luteus/Pinus sylvestris mycorrhizas, collected from zinc wastes in Southern Poland, were selected as potential biofilters on the basis of earlier studies carried out with energy dispersive spectrometry (EDS) microanalytical system coupled to scanning electron microscope (SEM) and transmission electron microscope (TEM). Using the National Accelerator Centre (NAC) nuclear microprobe, elemental concentrations in the ectomycorrhiza parts were for the first time estimated quantitatively. Micro-proton-induced X-ray emission (PIXE) true elemental maps from freeze-dried and chemically fixed mycorrhizas revealed strong accumulation of Ca, Fe, Zn and Pb within the fungal mantle and in the rhizomorph. Vascular tissue was enriched with P, S and K, while high concentrations of Si and Cl were present in the endodermis. Cu was the only element showing elevated concentrations in the cortex region. Elemental losses and redistributions were found in mycorrhizas prepared by chemical fixation. Some problems related to elemental imaging are discussed.
ISSN:0168-583X
1872-9584
DOI:10.1016/S0168-583X(01)00631-0