Using ATP bioluminescence technique for monitoring microbial activity in sludge

This study demonstrates, for the first time, that the presence of suspended solids in waste‐activated sludge interferes with adenosine triphosphate (ATP) bioluminescence tests. The sludge subject to acid/alkaline treatment represented the test sample. Without consideration of the effect of solid con...

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Bibliographic Details
Published in:Biotechnology and bioengineering 2001-11, Vol.75 (4), p.469-474
Main Authors: Chu, C. P., Lee, D. J., Chang, Bea-Ven, Liao, C. S.
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - analysis
Adenosine Triphosphate - metabolism
Adenosinetriphosphate
ATP
Bacteria
Bacteria - metabolism
Biological and medical sciences
Bioluminescence
Biomass
Biotechnology
Firefly Luciferin - metabolism
Fundamental and applied biological sciences. Psychology
Hydrogen-Ion Concentration
light blockage
Luciferases - metabolism
Luminescent Measurements
Mathematics
Methods. Procedures. Technologies
Microbiological Techniques - methods
Microbiology
Others
Sewage - microbiology
solids
Various methods and equipments
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Summary:This study demonstrates, for the first time, that the presence of suspended solids in waste‐activated sludge interferes with adenosine triphosphate (ATP) bioluminescence tests. The sludge subject to acid/alkaline treatment represented the test sample. Without consideration of the effect of solid concentrations, one would erroneously estimate the density levels of heterotrophic bacteria in the sludge using ATP data. A light blockage model was proposed to evaluate the luminescence reading without the interference of suspended solids. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 75: 469–474, 2001.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.10076