Enhanced chemiluminescence biosensor for the determination of phenolic compounds and hydrogen peroxide

The enhanced chemiluminescence (CL) reaction of the luminol–H 2O 2–horseradish peroxidase (HRP) system with immobilized HRP using microencapsulation in a sol–gel matrix has been used to develop a biosensor for p-iodophenol, p-coumaric acid, 2-naphthol and hydrogen peroxide. The detection limits obta...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2001-02, Vol.73 (1), p.71-75
Main Authors: Ramos, M.C, Torijas, M.C, Dı́az, A.Navas
Format: Article
Language:English
Subjects:
Biosensor
Chemiluminescence
Enhanced chemiluminescence
HRP
Hydrogen peroxide
Optical fibers
Optical fibre
Phenolic compounds
Phenols
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Summary:The enhanced chemiluminescence (CL) reaction of the luminol–H 2O 2–horseradish peroxidase (HRP) system with immobilized HRP using microencapsulation in a sol–gel matrix has been used to develop a biosensor for p-iodophenol, p-coumaric acid, 2-naphthol and hydrogen peroxide. The detection limits obtained for p-iodophenol, p-coumaric acid and 2-naphthol were 0.83 μM, 15 and 48 nM, respectively. Direct enzyme immobilization onto the end of the optical fibre permits the construction of a remote enhanced CL biosensor. This remote biosensor has been applied to hydrogen peroxide assay (detection limit 52.2 μM and relative standard deviation 4.7% for n=4) and the results have been compared with the biosensor developed in cuvette.
ISSN:0925-4005
1873-3077
DOI:10.1016/S0925-4005(00)00681-X