Proresolving Lipid Mediators Resolvin D1 and Protectin D1 Isomer Attenuate Neointimal Hyperplasia in the Rat Carotid Artery Balloon Injury Model

• Published in: The Journal of surgical research 2019-01, Vol.233, p.104-110
• Main Authors: Makino, Yoshihisa, Miyahara, Takuya, Nitta, Jun, Miyahara, Kazuhiro, Seo, Akihiko, Kimura, Masaru, Suhara, Masamitsu, Akai, Atsushi, Akagi, Daisuke, Yamamoto, Kota, Hoshina, Katsuyuki
• Format: Article
• Language: English
• Subjects: Atherosclerosis; Inflammation; Neointimal hyperplasia; Protectin D1 isomer; Resolvin D1; ω-3 fatty acid
• ISSN: 0022-4804; 1095-8673
• DOI: 10.1016/j.jss.2018.07.049

Specialized proresolving mediators from ω-3 polyunsaturated fatty acid may control resolution of inflammation. We evaluated the influence of two specialized proresolving mediators, resolvin D1 (RvD1) and protectin D1 isomer (PD1 iso) on neointimal hyperplasia after balloon injury. Sprague Dawley male rats at 12-14 wk of age were injured as a model of balloon angioplasty. Then, 1 μg/rat of RvD1 or PD1 iso was administered intravenously via the tail vein immediately and 2 d after angioplasty. The proliferation of injured artery and the infiltration of leukocytes, monocytes, and macrophages at 3 d after injury were evaluated by immunostaining. The activity of the inflammatory transcription factor nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB) in the injured artery at 3 d after injury was evaluated using an enzyme-linked immuno sorbent assay kit. The proliferation of the neointima was evaluated by calculating the ratio of the neointimal and medial areas using specimens at 14 d after injury. RvD1 and PD1 iso attenuated proliferation of medial cells (P