Isolation of an algicidal bacterium and its effects against the harmful-algal- bloom dinoflagellate Prorocentrum donghaiense (Dinophyceae)

• Published in: Harmful algae 2018-12, Vol.80, p.72-79
• Main Authors: Shi, Xinguo, Liu, Lemian, Li, Yue, Xiao, Yuchun, Ding, Guangmao, Lin, Senjie, Chen, Jianfeng
• Format: Article
• Language: English
• Subjects: Algicidal bacteria; Alteromonas; Beta-glucosidases; Cell wall polysaccharides; Prorocentrum donghaiense
• ISSN: 1568-9883; 1878-1470
• DOI: 10.1016/j.hal.2018.09.003

•A bacterium (FDHY-03) was isolated in a bloom of Prorocentrum donghaiense.•FDHY-03 strain showed high algicidal rate against P. donghaiense.•FDHY-03 lysed P. donghaiense cells from megacytic growth zone.•FDHY-03 lysed P. donghaiense cells by digesting cell wall polysaccharides. The relationship between algicidal bacteria and harmful-algal-bloom-forming dinoflagellates is understudied and their action modes are largely uncharacterized. In this study, an algicidal bacterium (FDHY-03) was isolated from a bloom of Prorocentrum donghaiense and the characteristics of its action against P. donghaiense was investigated at physiological, molecular, biochemical and cytological levels. 16S rDNA sequence analysis placed this strain in the genus of Alteromonas in the subclass of γ-proteobacteria. Algicidal activity was detected in the bacterial filtrate, suggesting a secreted algicidal principle from this bacterium. Strain FDHY-03 showed algicidal activity on a broad range of HAB-forming species, but the greatest effect was found on P. donghaiense, which showed 91.7% mortality in 24 h of challenge. Scanning electron microscopic analysis indicated that the megacytic growth zone of P. donghaiense cells was the major target of the algicidal action of FDHY-03. When treated with FDHY-03 culture filtrate, P. donghaiense cell wall polysaccharides decreased steadily, suggesting that the algicidal activity occurred through the digestion of cell wall polysaccharides. To verify this proposition, the expression profile of beta-glucosidase gene in FDHY-03 cultures with or without P. donghaiense cell addition was investigated using reverse-transcription quantitative PCR. The gene expression level increased in the presence of P. donghaiense cells, indicative of beta-glucosidase induction by P. donghaiense and the enzyme’s role in this dinoflagellate’s demise. This study has isolated a new bacterial strain with a strong algicidal capability, documented its action mode and biochemical mechanism, providing a potential source of bacterial agent to control P. donghaiense blooms.