Preparation of Bacterial DNA Template by Boiling and Effect of Immunoglobulin G as an Inhibitor in Real-Time PCR for Serum Samples from Patients with Brucellosis

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Published in:Clinical and Vaccine Immunology 2008-02, Vol.15 (2), p.293-296
Main Authors: Queipo-Ortuño, Maria Isabel, De Dios Colmenero, Juan, Macias, Manuel, Bravo, Maria Jose, Morata, Pilar
Format: Article
Language:English
Subjects:
Bacteria
Blotting, Western
Brucellosis - diagnosis
Clinical and Diagnostic Laboratory Immunology
DNA, Bacterial - genetics
DNA, Bacterial - isolation & purification
Electrophoresis, Polyacrylamide Gel
Enzyme Inhibitors - pharmacology
Humans
Immunoglobulin G - pharmacology
Polymerase Chain Reaction - methods
Serum - chemistry
Serum - microbiology
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Summary:Classifications Services CVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue CVI About CVI Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy CVI RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 1556-6811 Online ISSN: 1556-679X Copyright © 2014 by the American Society for Microbiology.   For an alternate route to CVI .asm.org, visit: CVI       
ISSN:1556-6811
1556-679X
1556-679X
DOI:10.1128/CVI.00270-07