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Horseradish peroxidase sol–gel immobilized for chemiluminescence measurements of alkaline-phosphatase activity
2-Naphthyl phosphate is a pro-enhancer of the luminol–H 2O 2–horseradish peroxidase reaction. Alkaline-phosphatase hydrolyses the phosphate group and produces 2-naphthol. This compound is an enhancer of the chemiluminescence (CL) reaction. The influence of 2-naphthyl phosphate and the incubation tim...
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Published in: | Sensors and actuators. B, Chemical Chemical, 2002-02, Vol.82 (2), p.176-179 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | 2-Naphthyl phosphate is a pro-enhancer of the luminol–H
2O
2–horseradish peroxidase reaction. Alkaline-phosphatase hydrolyses the phosphate group and produces 2-naphthol. This compound is an enhancer of the chemiluminescence (CL) reaction. The influence of 2-naphthyl phosphate and the incubation time on the chemiluminescence reaction, have been studied. The horseradish peroxidase (HRP) was immobilized in a sol–gel matrix, obtaining a biosensor for alkaline-phosphatase. The relative standard deviations using free HRP in solution and the immobilized one were 3.2 (
n=4) and 9.3% (
n=3), respectively. The detection limits for alkaline-phosphatase using free and immobilized HRP were 128 and 106
μU/ml, respectively. |
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ISSN: | 0925-4005 1873-3077 |
DOI: | 10.1016/S0925-4005(01)01003-6 |