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Horseradish peroxidase sol–gel immobilized for chemiluminescence measurements of alkaline-phosphatase activity

2-Naphthyl phosphate is a pro-enhancer of the luminol–H 2O 2–horseradish peroxidase reaction. Alkaline-phosphatase hydrolyses the phosphate group and produces 2-naphthol. This compound is an enhancer of the chemiluminescence (CL) reaction. The influence of 2-naphthyl phosphate and the incubation tim...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2002-02, Vol.82 (2), p.176-179
Main Authors: Dı́az, A.Navas, Sánchez, F.Garcı́a, Ramos, M.C, Torijas, M.C
Format: Article
Language:English
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Summary:2-Naphthyl phosphate is a pro-enhancer of the luminol–H 2O 2–horseradish peroxidase reaction. Alkaline-phosphatase hydrolyses the phosphate group and produces 2-naphthol. This compound is an enhancer of the chemiluminescence (CL) reaction. The influence of 2-naphthyl phosphate and the incubation time on the chemiluminescence reaction, have been studied. The horseradish peroxidase (HRP) was immobilized in a sol–gel matrix, obtaining a biosensor for alkaline-phosphatase. The relative standard deviations using free HRP in solution and the immobilized one were 3.2 ( n=4) and 9.3% ( n=3), respectively. The detection limits for alkaline-phosphatase using free and immobilized HRP were 128 and 106 μU/ml, respectively.
ISSN:0925-4005
1873-3077
DOI:10.1016/S0925-4005(01)01003-6