Diversity and seasonal distribution of ammonia-oxidizing archaea in the water column of a tropical estuary along the southeast Arabian Sea

• Published in: World journal of microbiology & biotechnology 2018-12, Vol.34 (12), p.188-188, Article 188
• Main Authors: Vipindas, Puthiya Veettil, Jabir, Thajudeen, Jasmin, Chekidhenkuzhiyil, Balu, Tharakan, Rehitha, Thekkendavida Velloth, Adarsh, Balakrishnan Meenakshikutty, Nair, Shanta, Abdulla, Mohamed Hatha, Abdulaziz, Anas
• Format: Article
• Language: English
• Subjects: Ammonia - metabolism; Applied Microbiology; Archaea - classification; Archaea - genetics; Archaea - metabolism; Bacteria - classification; Bacteria - genetics; Bacteria - metabolism; Biochemistry; Biodiversity; Biomedical and Life Sciences; Biotechnology; DNA, Archaeal; DNA, Bacterial - genetics; Environment; Environmental Engineering/Biotechnology; Estuaries; Genes, Archaeal - genetics; Geologic Sediments - microbiology; India; Life Sciences; Microbiology; Original Paper; Phylogeny; RNA, Ribosomal, 16S - genetics; Seasons; Seawater - microbiology; Sequence Analysis, DNA; Tropical Climate
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-018-2570-0

Diversity and distribution pattern of ammonia-oxidizing archaea (AOA) were studied across a salinity gradient in the water column of Cochin Estuary (CE), a tropical monsoonal estuary along the southeast Arabian Sea. The water column of CE was found to be nutrient rich with high bacterial (3.7–6.7 × 10 8  cells L −1 ) and archaeal abundance (1.9–4.5 × 10 8  cells L −1 ). Diversity and seasonal variation in the distribution pattern of AOA were studied using clone library analysis and Denaturing gradient gel electrophoresis (DGGE). Clone library analysis of both the amoA and 16S rRNA gene sequences showed similar diversity pattern, however the diversity was more clear when the 16S rRNA gene sequences were analyzed. More than 70% of the sequences retrieved were clustered under uncultured Thaumarchaeota group 1 lineage and the major fractions of the remaining sequences were grouped into the Nitrosopumilus lineage and Nitrosopelagicus lineage. The AOA community in the CE was less adaptable to changing environmental conditions and its distribution showed seasonal variations within the DGGE banding pattern with higher diversity during the pre-monsoon period. The distribution of AOA also showed its preference to intermediate salinity for their higher diversity. Summer monsoon associated runoff and flushing played a critical role in regulating the seasonality of AOA distribution.