Effect of DNA damage of cumulus oophorus cells and lymphocytes analyzed by alkaline comet assay on oocyte quality and intracytoplasmic sperm injection success among patients with polycystic ovary syndrome

Aim Cumulus oophorus cells (COC) exhibit a close relationship with the oocytes. We aimed to determine the role of the DNA integrity of COC and lymphocytes on the oocyte and embryo quality and intracytoplasmic sperm injection (ICSI) success. Methods The COC obtained with the mechanical denudation of...

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Bibliographic Details
Published in:The journal of obstetrics and gynaecology research 2019-03, Vol.45 (3), p.609-618
Main Authors: Tola, Esra N., Koşar, Pınar A., Karatopuk, Dilek U., Sancer, Okan, Oral, Baha
Format: Article
Language:English
Subjects:
Body mass index
Comet assay
cumulus oophorus cells
Deoxyribonucleic acid
DNA
DNA damage
DNA integrity parameters
Embryos
Fertilization
Injection
intracytoplasmic sperm injection
Lymphocytes
oocyte quality
Oocytes
Polycystic ovary syndrome
Pregnancy
Quality
Sperm
Success
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Summary:Aim Cumulus oophorus cells (COC) exhibit a close relationship with the oocytes. We aimed to determine the role of the DNA integrity of COC and lymphocytes on the oocyte and embryo quality and intracytoplasmic sperm injection (ICSI) success. Methods The COC obtained with the mechanical denudation of each oocyte and the lymphocytes obtained on oocyte retrieval day from 69 infertile polycystic ovary syndrome (PCOS) patients were used. The tail length, tail moment and tail DNA percentage were evaluated using an alkaline comet assay. The oocytes and embryos were graded. Clinical pregnancy was defined as the presence of a gestational sac with a beating heart. Results All of the DNA integrity parameters of the COC and lymphocytes were similar between the good and poor quality oocytes and between the good and poor quality embryos. There was no relationship between the DNA damage parameters and the fertilization and clinical pregnancy. The day 2 hormone levels, body mass index and age were negatively correlated with the DNA integrity parameters. No links were found between the embryo and oocyte scores and the DNA damage parameters. Conclusion The DNA integrity of the COC and lymphocytes did not seem to be related to the oocyte and embryo quality and ICSI success.
ISSN:1341-8076
1447-0756
DOI:10.1111/jog.13868