Mitochondrial aldehyde dehydrogenase 2 protects against high glucose-induced injury in neonatal rat cardiomyocytes by regulating CaN-NFAT3 signaling pathway

To investigate whether CaN-NFAT3 pathway mediates the protective effects of aldehyde dehydrogenase (ALDH) 2 in high glucose-treated neonatal rat ventricular myocytes. The ventricular myocytes were isolated from the heart of neonatal (within 3 days) SD rats by enzyme digestion and cultured in the pre...

Full description

Saved in:
Bibliographic Details
Published in:Nan fang yi ke da xue xue bao = Journal of Southern Medical University 2018-11, Vol.38 (11), p.1288-1293
Main Authors: Guo, Jianlu, Kang, Pinfang, Zhu, Lei, Sun, Shuo, Tao, Min, Zhang, Heng, Tang, Bi
Format: Article
Language:Chinese
Subjects:
Aldehyde Dehydrogenase, Mitochondrial - antagonists & inhibitors
Aldehyde Dehydrogenase, Mitochondrial - metabolism
Animals
Animals, Newborn
Benzamides - pharmacology
Benzodioxoles - pharmacology
Calcium - metabolism
Cells, Cultured
Culture Media
Enzyme Inhibitors - pharmacology
Glucose - administration & dosage
Glucose - pharmacology
Isoflavones - pharmacology
Mitochondria, Heart - enzymology
Myocytes, Cardiac - drug effects
Myocytes, Cardiac - metabolism
NFATC Transcription Factors - metabolism
Nuclear Pore Complex Proteins - metabolism
Rats
Rats, Sprague-Dawley
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To investigate whether CaN-NFAT3 pathway mediates the protective effects of aldehyde dehydrogenase (ALDH) 2 in high glucose-treated neonatal rat ventricular myocytes. The ventricular myocytes were isolated from the heart of neonatal (within 3 days) SD rats by enzyme digestion and cultured in the presence of 5-Brdu. After reaching confluence, the cultured ventricular myocytes were identified using immunofluorescence assay for -SA protein. The cells were then cultured in either normal (5 mmol/L) or high glucose (30 mmol/L) medium in the presence of ALDH2 agonist Alda-1, ALDH 2 inhibitor Daidzin, or Alda-1 and NFAT3 inhibitor (11R-VIVIT). Fluorescent probe and ELISA were used to detect intracellular Ca concentration and CaN content, respectively; ALDH2, CaN and NFAT3 protein expressions in the cells were detected using Western blotting. Compared with cells cultured in normal glucose, the cells exposed to high glucose showed a significantly decreased expression of ALDH2 protein ( < 0.05) and increased expressions
ISSN:1673-4254
DOI:10.12122/j.issn.1673-4254.2018.11.02