TaqMan-Based Real-Time PCR Method for Detection of Yersinia pseudotuberculosis in Food

A sensitive and specific assay for detection of food-borne pathogenic Yersinia pseudotuberculosis was developed. The primer-probe set was designed to target a 157-bp sequence of the chromosomally located gene ail. The complete method, including an internal amplification control, was evaluated for se...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2008-10, Vol.74 (20), p.6465-6469
Main Authors: Lambertz, S. Thisted, Nilsson, C, Hallanvuo, S
Format: Article
Language:English
Subjects:
Bacteria
Base Sequence
Biological and medical sciences
Chromosomes
DNA Primers - genetics
Food Microbiology
Fundamental and applied biological sciences. Psychology
Genes
Genes, Bacterial
Methods
Microbiology
Molecular Sequence Data
Polymerase Chain Reaction - methods
Polymerase Chain Reaction - standards
Reference Standards
Sensitivity and Specificity
Yersinia pseudotuberculosis
Yersinia pseudotuberculosis - genetics
Yersinia pseudotuberculosis - isolation & purification
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Description
Summary:A sensitive and specific assay for detection of food-borne pathogenic Yersinia pseudotuberculosis was developed. The primer-probe set was designed to target a 157-bp sequence of the chromosomally located gene ail. The complete method, including an internal amplification control, was evaluated for several different food items.
ISSN:0099-2240
1098-5336
1098-6596
DOI:10.1128/AEM.01459-08