Histamine, Cadaverine, and Putrescine Produced In Vitro by Enterobacteriaceae and Pseudomonadaceae Isolated from Spinach

A total of 364 bacterial isolates, obtained from spinach leaves, were assayed in a decarboxylase broth containing histidine, lysine, and ornithine to check their ability to produce biogenic amines, and then quantified by high-performance liquid chromatography. Among these isolates, 240 formed cadave...

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Bibliographic Details
Published in:Journal of food protection 2010-02, Vol.73 (2), p.385-389
Main Authors: LAVIZZARI, T, BRECCIA, M, BOVER-CID, S, VIDAL-CAROU, M. C, VECIANA-NOGUES, M. T
Format: Article
Language:English
Subjects:
Amines
Amino acids
Bacteria
Biogenic Amines - biosynthesis
Biological and medical sciences
Cadaverine - biosynthesis
Chromatography
Chromatography, High Pressure Liquid
Enterobacter amnigenus
Enterobacter cloacae
Enterobacteriaceae
Enterobacteriaceae - isolation & purification
Enterobacteriaceae - metabolism
Flowers & plants
Food
Food Contamination - analysis
Food industries
Food microbiology
Food safety
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
Hafnia alvei
Histamine
Histamine - biosynthesis
Klebsiella pneumoniae
Liquid chromatography
Microbiology
Microorganisms
Morganella morganii
Pseudomonadaceae
Pseudomonadaceae - isolation & purification
Pseudomonadaceae - metabolism
Putrescine - biosynthesis
Serratia liquefaciens
Serratia marcescens
Species Specificity
Spinach
Spinacia oleracea
Spinacia oleracea - microbiology
Stenotrophomonas maltophilia
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Summary:A total of 364 bacterial isolates, obtained from spinach leaves, were assayed in a decarboxylase broth containing histidine, lysine, and ornithine to check their ability to produce biogenic amines, and then quantified by high-performance liquid chromatography. Among these isolates, 240 formed cadaverine, 208 formed putrescine, and 196 formed histamine, in widely varying amounts. They frequently produced more than one biogenic amine. Klebsiella pneumoniae subsp. pneumoniae and Morganella morganii were the main histamine producers, with mean values of 1,600 and 2,440 mg/liter, respectively, followed by Pantoea spp. 3 (1,710 mg/liter) and Hafnia alvei (2,500 mg/liter). Enterobacter amnigenus and Enterobacter cloacae produced particularly high amounts of putrescine, with mean values of 2,340 and 2,890 mg/liter, respectively. The strongest cadaverine formation was shown by Serratia liquefaciens (3,300 mg/liter), Serratia marcescens (3,280 mg/liter), and Stenotrophomonas maltophilia (1,000 mg/liter).
ISSN:0362-028X
1944-9097
DOI:10.4315/0362-028X-73.2.385