TroCCL4, a CC chemokine of Trachinotus ovatus, is involved in the antimicrobial immune response

• Published in: Fish & shellfish immunology 2019-03, Vol.86, p.525-535
• Main Authors: Sun, Baiming, Lei, Yang, Cao, Zhenjie, Zhou, Yongcan, Sun, Yun, Wu, Ying, Wang, Shifeng, Guo, Weiliang, Liu, Chunsheng
• Format: Article
• Language: English
• Subjects: Antimicrobial function; CC chemokines; Migration; Proliferation; Trachinotus ovatus
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2018.11.080

CC chemokines are a large subfamily of chemokines that play an important role in the innate immune system. To date, several CC chemokines have been identified in fish species; however, the activities and functions of these putative chemokines remain ambiguous in teleosts, especially in the golden pompano, Trachinotus ovatus. Here, we characterized CC chemokine ligand 4 from T. ovatus (TroCCL4) and studied its functions. TroCCL4 contains a 294 bp open reading frame that encodes a putative peptide comprising 97 amino acids. TroCCL4 shares a high amino acid sequence similarity of 31.11%–78.35% with other CC chemokines sequences in humans and teleosts and has four cysteine residues that are conserved among other CC chemokines. TroCCL4 is also related to the macrophage inflammatory protein (MIP) group of CC chemokines. TroCCL4 expression was most abundant in immune organs and significantly upregulated in a time-dependent manner following Edwardsiella tarda infection. Recombinant TroCCL4 (rTroCCL4) induced the migration of peripheral blood leukocytes and the cellular proliferation of head kidney lymphocytes. In addition, rTroCCL4 inhibited the growth of Escherichia coli and E. tarda, indicating an antimicrobial function. Furthermore, the results of in vivo analysis showed that TroCCL4 overexpression in T. ovatus significantly enhanced macrophage activation; upregulated the gene expression of interleukin 1-β (IL-1β), interleukin 15 (IL15), interferon-induced Mx protein (Mx), tumor necrosis factor α (TNFα), complement C3, and major histocompatibility complex (MHC) class Iα and class IIα; and protected against bacterial infection in fish tissues. In contrast, knockdown of TroCCL4 expression resulted in increased bacterial dissemination and colonization in fish tissues. Taken together, our results provide evidence indicating that TroCCL4 has the ability to stimulate leukocytes and macrophages and enhance host immunity to defend against bacterial infection. •A CC chemokine ligand 4 in Trachinotus ovatus (TroCCL4) was identified and characterized.•TroCCL4 could induce PBL migration and lymphocyte proliferation, inhibit bacteria growth, and induce macrophage activation.•The in vivo and in vitro studies demonstrated that TroCCL4 was involved in antibacterial immunity.