An in vitro study of the protective effect of caffeic acid on human erythrocytes

The interaction and protective effect of caffeic acid (CA) on human erythrocytes (RBC) and molecular models of its membrane were studied. The latter consisted of bilayers built up of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylethanolamine (DMPE), representative of phospholipid...

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Bibliographic Details
Published in:Archives of biochemistry and biophysics 2019-02, Vol.662, p.75-82
Main Authors: Colina, José R., Suwalsky, Mario, Manrique-Moreno, Marcela, Petit, Karla, Aguilar, Luis F., Jemiola-Rzeminska, Malgorzata, Strzalka, Kazimierz
Format: Article
Language:English
Subjects:
Antioxidant
Caffeic acid
Caffeic Acids - pharmacology
Calorimetry, Differential Scanning
Dimyristoylphosphatidylcholine - metabolism
Erythrocyte membrane
Erythrocytes - drug effects
Erythrocytes - metabolism
Glycerophospholipids - metabolism
Hemolysis - drug effects
Humans
In Vitro Techniques
Lipid bilayer
Microscopy, Electron, Scanning
Spectrometry, Fluorescence
X-Ray Diffraction
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Summary:The interaction and protective effect of caffeic acid (CA) on human erythrocytes (RBC) and molecular models of its membrane were studied. The latter consisted of bilayers built up of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylethanolamine (DMPE), representative of phospholipid classes located in the outer and inner monolayers of the human erythrocyte membrane, respectively. X-ray diffraction and differential scanning calorimetry results indicated that CA induced structural and thermotropic perturbations in multilayers and vesicles of DMPC. Fluorescence spectroscopy analysis showed that CA increased the fluidity of DMPC vesicles and of human erythrocyte ghosts. Scanning electron microscopy observations displayed that CA induced morphological alterations to RBC from their normal discoid form to echinocytes. The assessment of its protective capacity showed that CA inhibits RBC morphological alterations and lysis induced by HClO. These findings imply that CA molecules were located in the outer monolayer of the erythrocyte membrane, and that this preferential location might effectively protect the red cells from damage caused by oxidizing species.
ISSN:0003-9861
1096-0384
DOI:10.1016/j.abb.2018.12.006