Anti-arthritic effect of pentosan polysulfate in rats with collagen-induced arthritis

• Published in: Research in veterinary science 2019-02, Vol.122, p.179-185
• Main Authors: Suranji Wijekoon, H.M., Kim, Sangho, Bwalya, Eugene C., Fang, Jing, Aoshima, Keisuke, Hosoya, Kenji, Okumura, Masahiro
• Format: Article
• Language: English
• Subjects: Acid phosphatase; Acid phosphatase (tartrate-resistant); Acid resistance; Ankle; Arthritis; Biocompatibility; Bioindicators; Biomarkers; Biomedical materials; Cartilage; Cartilage oligomeric matrix protein; Cathepsin K; Collagen; Collagen (type II); Collagen-induced arthritis; Cytokines; Disease-modifying antiarthritic agent; Drug dosages; Edema; Freund's incomplete adjuvant; IL-1β; Inflammation; Interleukins; Matrix protein; Meloxicam; mRNA; Nonsteroidal anti-inflammatory drugs; Pentosan polysulfate; Proteins; Rats; Recovery of function; Studies; Synovial fluid; Synovial membrane; Tumor necrosis factor-α; Veterinary medicine
• ISSN: 0034-5288; 1532-2661
• DOI: 10.1016/j.rvsc.2018.11.028

Pentosan polysulfate (PPS) is currently under investigation as a potential disease-modifying antiarthritic agent. In the present study the effects of PPS on arthritic profiles based on clinical score, ankle size, histological changes, and activity of inflammatory mediators using collagen-induced arthritic rat are reported. Model of arthritis was developed in Sprague Dawley rats by intradermal injection of bovine type II collagen emulsified with incomplete Freund's adjuvant. The rats were randomly divided into four groups: normal control, arthritic control, arthritic rats treated with PPS (at dose level 20 μg/g) and arthritic rats treated with meloxicam (2 μg/g). The treatment was continued daily until the day 30. Arthritic biomarkers (cartilage oligomeric matrix protein and tartrate-resistant acid phosphatase 5b) in synovial fluid, expression of inflammatory mediators (interleukin-1β, and tumor necrosis factor-α) and osteoclast marker genes (cathepsin K, tartrate-resistant acid phosphatase) in synovial membrane were measured. Daily administration of PPS to the arthritic rats significantly decreased the severity of arthritis by effectively suppressing the symptoms of arthritis and improving the functional recovery based on clinical score and histopathological evidence. Intriguingly, identical downregulation pattern of arthritis profiles, biological markers as well as relative mRNA levels of osteoclast markers and cytokines were monitored in arthritic rats treated with PPS. In conclusion, PPS exerted protective effects against collagen-induced arthritis in rats. The results suggest that PPS acts as an anti-inflammatory and anti-arthritic agent in decreasing the arthritic effects in collagen-induced arthritic rats. •Pentosan polysulfate possesses anti-arthritic effects on CIA rats by ameliorating clinical and functional outcome.•PPS inhibits the enzymes that degrade the extra-cellular matrix and cause joint destruction in inflammatory arthritis.•PPS preserves the nearly normal histological architecture by restoring the degraded articular cartilage in CIA rats.