Evaluation of levels of interleukin-6, interleukin-8 and some haematologic parameters of dogs with cutaneous wounds

• Published in: Cytokine (Philadelphia, Pa.) Pa.), 2019-01, Vol.113, p.128-138
• Main Authors: Avazi, Daniel Onimisi, Awasum, Augustine Cheh, Hassan, Adamu Zoaka, Ayo, Joseph Olusegun, Aluwong, Tagang, Muhammed, Sa'idu Tanko, Simon, Ayo Yila, Suleiman, Mohammad Hadi, Kudi, Ayuba Caleb
• Format: Article
• Language: English
• Subjects: Animals; Dog; Dogs; Female; Haematology; Hematology - methods; Inflammation; Interleukin-6; Interleukin-6 - blood; Interleukin-8; Interleukin-8 - blood; Male; Skin Diseases - blood; Tumor Necrosis Factor-alpha - blood; Wound; Wound Healing - physiology; Wounds and Injuries - blood
• ISSN: 1043-4666; 1096-0023
• DOI: 10.1016/j.cyto.2018.06.024

•To investigate the time-course of serum and wound fluid IL-6 and IL-8 levels and specific hematologic parameters.•Wound fluid and serum IL-6 peaked 12 hours post-surgery.•IL-8 was significantly higher in serum than wound fluid.•IL-6 and IL-8 modulated cutaneous wound healing processes. The aim of the study was to investigate the time-course of serum and wound fluids interleukin (IL)-6 and IL-8 levels in dogs with cutaneous wounds and their relationship with some haematologic parameters. The experimental group comprised of six adult dogs that underwent surgery with wounds (n = 6) on the mid lateral aspect of the right antebrachium; and control group of six, apparently, healthy intact (free from cutaneous wounds) adult dogs, comprising equal number of both sexes. Vital signs evaluated were within normal limits. Samples of blood, serum and wound fluids harvested pre- and at 12 h, 36 h, 60 h, 156 h and 324 h post-injury, were utilised for IL-6 and IL-8 assay and haematology. Peak concentrations of IL-6 in wound fluid (1.33. ± 0.33 ng/mL) and serum (0.82 ± 0.24 ng/mL) of the experimental group at 12 h post-operation were higher (P