A Potential Role for GSK3β in Glucose-Driven Intrauterine Catch-Up Growth in Maternal Obesity

• Published in: Endocrinology (Philadelphia) 2019-02, Vol.160 (2), p.377-386
• Main Authors: Appel, Sarah, Grothe, Jon, Storck, Sarah, Janoschek, Ruth, Bae-Gartz, Inga, Wohlfarth, Maria, Handwerk, Marion, Hucklenbruch-Rother, Eva, Gellhaus, Alexandra, Dötsch, Jörg
• Format: Article
• Language: English
• Subjects: Birth weight; Diet; Endocrinology; Fetuses; Glucose; Glucose transporter; Glycogen; Glycogens; High fat diet; Nutrition; Obesity; Offspring; Phenotypes; Placenta; Placental transfer; Pregnancy; Prenatal development; Protein transport; Weaning; Wnt protein
• ISSN: 1945-7170; 0013-7227; 1945-7170
• DOI: 10.1210/en.2018-00899

Abstract Obesity and unhealthy nutrition are increasing and affect women of childbearing age and hence during pregnancy. Despite normal or even high birth weight, the offspring suffers from long-term metabolic risks. We hypothesized that fetal growth is disturbed during different intrauterine phases. Underlying molecular events remain elusive. Female mice were fed either a standard diet (SD) or a high-fat diet (HFD) after weaning until mating and during pregnancy. Pregnant mice were euthanized at gestational day (G)15.5 and G18.5, and fetuses and placentas were removed for analysis. HFD fetuses displayed intrauterine growth restriction (IUGR) at G15.5, which disappeared until G18.5, indicating intrauterine catch-up growth during that time period. Main placental findings indicate decreased canonical Wnt-GSK3β signaling and lower proliferation rates at G18.5, which goes along with a smaller placental transfer zone. On the other hand, glucose depots (glycogen cluster) in HFD placentas decreased more strongly between G15.5 and G18.5 compared with placentas from SD mothers, and the glucose transporter protein GLUT-1 was increased at G18.5 in the HFD group. Maternal diet-induced obesity causes an IUGR phenotype at the beginning of the third week (G15.5) in our mouse model. This phenotype is reversed by the end of the third week (G18.5) despite a smaller placental transfer zone, probably based on GSK3β-mediated increased glucose mobilization in the placenta and hence an increased glucose supply to the fetus.