ATP Synthase Subunit Beta Immunostaining is Reduced in the Sclerotic Hippocampus of Epilepsy Patients

• Published in: Cellular and molecular neurobiology 2019-01, Vol.39 (1), p.149-160
• Main Authors: Mota, Marcelo Vilas Boas, Zaidan, Bruna Cunha, do Canto, Amanda Morato, Ghizoni, Enrico, Tedeschi, Helder, de Souza Queiroz, Luciano, Alvim, Marina K. M., Cendes, Fernando, Lopes-Cendes, Iscia, Schenka, André Almeida, Vieira, André Schwambach, Rogerio, Fabio
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; ATP synthase; Biomedical and Life Sciences; Biomedicine; Cell Biology; Cell Count; Convulsions & seizures; Dentate gyrus; Dentate Gyrus - pathology; Electrical stimuli; Epilepsy; Epilepsy - enzymology; Epilepsy - pathology; Excitability; Female; Hippocampus; Hippocampus - enzymology; Hippocampus - pathology; Humans; Immunohistochemistry; Male; Mass spectroscopy; Middle Aged; Mitochondria; Mitochondrial Proton-Translocating ATPases - metabolism; Necropsy; Neurobiology; Neurons - metabolism; Neurons - pathology; Neurosciences; Original Research; Patients; Proteins; Proteomics; Sclerosis; Sclerosis - enzymology; Sclerosis - pathology; Seizures; Sodium; Sodium-Potassium-Exchanging ATPase; Surgery; Temporal lobe; Young Adult
• ISSN: 0272-4340; 1573-6830; 1573-6830
• DOI: 10.1007/s10571-018-0641-2

Epilepsy is a common disease presenting with recurrent seizures. Hippocampal sclerosis (HS) is the commonest histopathological alteration in patients with temporal lobe epilepsy (TLE) undergoing surgery. HS physiopathogenesis is debatable. We have recently studied, by using mass spectrometry-based proteomics, an experimental model of TLE induced by electrical stimulation. Specifically, protein expressions of both the beta subunit of mitochondrial ATP synthase (ATP5B) and of membrane ATPases were found to be reduced. Here, we investigated tissue distribution of ATP5B and sodium/potassium-transporting ATPase subunit alpha-3 (NKAα3), a protein associated with neuromuscular excitability disorders, in human hippocampi resected “en bloc” for HS treatment ( n  = 15). We used immunohistochemistry and the stained area was digitally evaluated (increase in binary contrast of microscopic fields) in the hippocampal sectors (CA1–CA4) and dentate gyrus. All HS samples were classified as Type 1, according to the International League Against Epilepsy (ILAE) 2013 Classification (predominant cell loss in CA1 and CA4). ATP5B was significantly decreased in all sectors and dentate gyrus of HS patients compared with individuals submitted to necropsy and without history of neurological alterations ( n  = 10). NKAα3 expression showed no difference. Moreover, we identified a negative correlation between frequency of pre-operative seizures and number of neurons in CA1. In conclusion, our data showed similarity between changes in protein expression in a model of TLE and individuals with HS. ATP5B reduction would be at least in part due to neuronal loss. Future investigations on ATP5B activity could provide insights into the process of such cell loss.