Clopidogrel reduces lipopolysaccharide‐induced inflammation and neutrophil‐platelet aggregates in an experimental endotoxemic model

Platelet activation contributes to organs failure in inflammation and plays an important role in endotoxemia. Clopidogrel inhibits platelet aggregation and activation. However, the role of clopidogrel in modulating inflammatory progression of endotoxemia remains largely unexplored. Therefore, we inv...

Full description

Saved in:
Bibliographic Details
Published in:Journal of biochemical and molecular toxicology 2019-04, Vol.33 (4), p.e22279-n/a
Main Authors: Wang, Xiao‐Li, Deng, Hua‐Fei, Li, Ting, Miao, Shu‐Ying, Xiao, Zi‐Hui, Liu, Mei‐Dong, Liu, Ke, Xiao, Xian‐Zhong
Format: Article
Language:English
Subjects:
Aggregates
Blood circulation
CD40L protein
Cell activation
Clopidogrel
Endotoxemia
Immunofluorescence
Inflammation
Leukocytes
Lipopolysaccharides
Lungs
Mice
Neutrophils
Organs
P2Y12
Platelet aggregation
platelets
Pretreatment
Thrombocytopenia
Tissue factor
Tissues
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Platelet activation contributes to organs failure in inflammation and plays an important role in endotoxemia. Clopidogrel inhibits platelet aggregation and activation. However, the role of clopidogrel in modulating inflammatory progression of endotoxemia remains largely unexplored. Therefore, we investigated the role of clopidogrel on the activation of platelet and leukocytes in lipopolysaccharide (LPS)‐induced inflammation in mice. Animals were treated with clopidogrel or vehicle before LPS induction. The expression of neutrophil‐platelet aggregates and platelet activation and tissue factor was determined. Immunofluorescence was used to analyze platelet‐leukocyte interactions and tissue factor (TF) expression on leukocytes. Clopidogrel pretreatment markedly decreased lung damage, inhibited platelet‐neutrophil aggregates and TF expression. In addition, clopidogrel reduced thrombocytopenia and affected the number of circulating white blood cell in endotoxemia mice. Moreover, clopidogrel also reduced platelet shedding of CD40L and CD62P in endotoxemic mice. Taken together, clopidogrel played an important role through reducing platelet activation and inflammatory process in endotoxemia.
ISSN:1095-6670
1099-0461
DOI:10.1002/jbt.22279