cAMP/PKA signalling pathway regulates redox homeostasis essential for Sporisorium scitamineum mating/filamentation and virulence

Summary The fungal pathogen Sporisorium scitamineum causes sugarcane smut disease. The formation and growth of dikaryotic hypha after sexual mating is critical for S. scitamineum pathogenicity, however regulation of S. scitimineum mating has not been studied in detail. We identified and characterize...

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Bibliographic Details
Published in:Environmental microbiology 2019-03, Vol.21 (3), p.959-971
Main Authors: Chang, Changqing, Cai, Enping, Deng, Yi Zhen, Mei, Dan, Qiu, Shanxu, Chen, Baoshan, Zhang, Lian‐Hui, Jiang, Zide
Format: Article
Language:English
Subjects:
Cyclic AMP
Cyclic AMP - metabolism
Cyclic AMP-Dependent Protein Kinases - metabolism
Filamentation
Fungi
Gene regulation
Genetics
Homeostasis
Intracellular
Mating
Oxidation-Reduction
Oxidoreductions
Pathogenicity
Pathogens
Plant Diseases - microbiology
Protein kinase A
Reactive oxygen species
Reactive Oxygen Species - metabolism
Reproductive behaviour
Saccharum - microbiology
Signal Transduction
Signaling
Smut
Sporisorium scitamineum
Sugarcane
Transcription
Ustilaginales - pathogenicity
Ustilaginales - physiology
Virulence
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Summary:Summary The fungal pathogen Sporisorium scitamineum causes sugarcane smut disease. The formation and growth of dikaryotic hypha after sexual mating is critical for S. scitamineum pathogenicity, however regulation of S. scitimineum mating has not been studied in detail. We identified and characterized the core components of the conserved cAMP/PKA pathway in S. scitamineum by reverse genetics. Our results showed that cAMP/PKA signalling pathway is essential for proper mating and filamentation, and thus critical for S. scitamineum virulence. We further demonstrated that an elevated intracellular ROS (reactive oxygen species) level promotes S. scitamineum mating‐filamentation, via transcriptional regulation of ROS catabolic enzymes, and is under regulation of the cAMP/PKA signalling pathway. Furthermore, we found that fungal cAMP/PKA signalling pathway is also involved in regulation of host ROS response. Overall, our work displayed a positive role of elevated intracellular ROS in fungal differentiation and virulence.
ISSN:1462-2912
1462-2920
DOI:10.1111/1462-2920.14496