Genetic variation of FTO: rs1421085 T>C, rs8057044 G>A, rs9939609 T>A, and copy number (CNV) in Mexican Mayan school‐aged children with obesity/overweight and with normal weight

• Published in: American journal of human biology 2019-01, Vol.31 (1), p.e23192-n/a
• Main Authors: González‐Herrera, Lizbeth, Zavala‐Castro, Jorge, Ayala‐Cáceres, Claudia, Pérez‐Mendoza, Gerardo, López‐González, María José, Pinto‐Escalante, Doris, Canto‐Cetina, Thelma, García‐Escalante, María Guadalupe, Rubi‐Castellanos, Rodrigo, Contreras‐Capetillo, Silvina, Herrera‐Sanchez, Fernando, Méndez‐Domínguez, Nina, Alcocer‐Gamboa, Alberto
• Format: Article
• Language: English
• Subjects: Adolescent; Alleles; Alpha-Ketoglutarate-Dependent Dioxygenase FTO - genetics; Body fat; Body mass; Body mass index; Body size; Body weight; Body Weight - genetics; Child; Child, Preschool; Children; Children & youth; Copy number; Cross-Sectional Studies; Female; Gene frequency; Genetic diversity; Genetic Variation; Genotyping; Girls; Humans; Indians, North American - statistics & numerical data; Male; Mexico - epidemiology; Minority & ethnic groups; Obesity; Overweight; Overweight - epidemiology; Overweight - genetics; Pediatric Obesity - epidemiology; Pediatric Obesity - genetics; Polymerase chain reaction; Polymorphism, Single Nucleotide; Regression analysis; Regression models; Risk analysis; Risk Factors; Single-nucleotide polymorphism
• ISSN: 1042-0533; 1520-6300
• DOI: 10.1002/ajhb.23192

Objectives Genetic variation of the fat mass and obesity associated gene (FTO) has been identified as a risk factor for obesity and obesity traits. Distribution of FTO single nutleotide polymorphisms (SNPs) rs1421085T>C, rs9939609T>A, rs8057044G>A and copy number variation (CNV) was evaluated in association with childhood obesity or overweight status in children with Mayan ethnicity. Methods We included 318 school‐aged children with obesity or overweight status (body mass index [BMI]: >85th percentile) and 303 children with normal weight (BMI: 15th‐85th percentile). Genotyping was performed using real‐time polymerase chain reaction (RT‐PCR) with TaqMan probes. The cross‐sectional study was carried out using univariate and multivariate logistic regression models adjusted for gender. Results FTO‐SNP rs1421085 showed significant differences between children with obesity and children with normal weight for the heterozygous genotype (P = 0.003) and for allele frequencies (P = 0.023). Adjusting by gender, significant differences were found in frequencies of the hetezygous genotype of SNPs rs9939609 (P = 0.023) and rs1421085 (P = 0.003) as well as in allele frequencies (P = 0.042 and P = 0.013, respectively) between girls with obesity and girls without obesity. In contrast, SNP rs8057044 was significantly different only between heterozygous overweight versus normal weight boys (P = 0.035) and for the allele frequency of rs8057044 (P = 0.021). The mean relative CNV was significantly higher in male overweight children than in boys with normal weight (P = 0.000). Conclusions The FTO SNP rs1421085 is a genetic factor associated with obesity in Mayan school‐aged children. FTO SNPs rs1421085 and rs9939609 affect genetic susceptibility for obesity only in girls, whereas, SNP rs8057044 and CNV are associated with overweight status only in boys.