Protein-rich extract of Musca domestica larvae alleviated metabolic disorder in STZ-induced type 2 diabetic rat model via hepatoprotective and pancreatic β-cell protective activities

• Published in: Journal of biosciences 2018-12, Vol.43 (5), p.969-983
• Main Authors: Mei, Hanfang, Xu, Jianhua, He, Yinru, Yang, Xia, Liu, Wenbin, Tian, Wei, Zeng, Yu, Zhu, Jiayong
• Format: Article
• Language: English
• Subjects: Adenosine kinase; Adenosine monophosphate; Adipose tissue; AMP; AMP-Activated Protein Kinases - genetics; AMP-Activated Protein Kinases - metabolism; Animals; Biomedical and Life Sciences; Biomedicine; Blood; Blood glucose; Blood Glucose - drug effects; Blood Glucose - metabolism; Catalase; Catalase - genetics; Catalase - metabolism; Cell Biology; Cholesterol; Complex Mixtures - isolation & purification; Complex Mixtures - pharmacology; Density; Diabetes; Diabetes mellitus; Diabetes Mellitus, Experimental - chemically induced; Diabetes Mellitus, Experimental - drug therapy; Diabetes Mellitus, Experimental - genetics; Diabetes Mellitus, Experimental - metabolism; Drinking - drug effects; Eating - drug effects; Food intake; Gene Expression Regulation; Glucose; Glucose transporter; Glucose Transporter Type 4 - genetics; Glucose Transporter Type 4 - metabolism; Hepatocytes; High density lipoprotein; Houseflies - chemistry; Hypoglycemic Agents - isolation & purification; Hypoglycemic Agents - pharmacology; Insulin; Insulin - blood; Insulin-Secreting Cells - drug effects; Insulin-Secreting Cells - metabolism; Insulin-Secreting Cells - pathology; Kinases; Larva - chemistry; Larvae; Life Sciences; Lipoproteins - blood; Liver; Liver - drug effects; Liver - metabolism; Liver - pathology; Low density lipoprotein; Male; Malondialdehyde; Malondialdehyde - antagonists & inhibitors; Malondialdehyde - metabolism; Metabolic disorders; Microbiology; Musca domestica; Muscles; Necrosis; Oxidative stress; Pancreas; Pharmacology; Plant Sciences; PPAR gamma - genetics; PPAR gamma - metabolism; Protein kinase; Proteins; Rats; Rats, Sprague-Dawley; Skeletal muscle; Streptozocin; Superoxide dismutase; Superoxide Dismutase-1 - genetics; Superoxide Dismutase-1 - metabolism; Triglycerides; Urine; Water intake; Water intakes; Zoology
• ISSN: 0250-5991; 0973-7138
• DOI: 10.1007/s12038-018-9804-z

The study was designed to explore the beneficial effect of Musca domestica larvae extract (MDLE) on a metabolic disorder using a diabetic rat model. Streptozotocin-induced diabetic rats were treated with or without MDLE. Blood glucose, insulin levels, lipid profiles, and oxidative stress markers were measured. The morphological changes in the pancreas and liver were determined, as well as insulin expression. The expression of glucose transporter 4 (GLUT4), phospho-adenosine monophosphate-activated protein kinase (p-AMPK)/total AMPK, superoxide dismutase 1 (SOD1), catalase (CAT), and peroxisome proliferator-activated receptor gamma (PPARγ) were detected. Compared with untreated diabetic rats, MDLE-treated rats had decreased urine volume, food intake, and water intake, along with significantly lower levels of blood glucose, malondialdehyde (MDA), plasma triglycerides, low-density lipoprotein (LDL), and total cholesterol. MDLE-treated rats also had higher levels of SOD activity, high-density lipoprotein (HDL), and insulin. MDLE treatment partially restored the β-cell population, improved the liver necrosis and islet cell damage, reversed the decreased expression of GLUT4, phospho-AMPK, SOD1, and CAT in the liver, skeletal muscle and pancreatic tissue, and also increased the expression of PPARγ in the liver and adipose tissue in diabetic rats. In conclusion, the obtained results suggest that MDLE could possibly be used pharmacologically as an adjuvant for the treatment of diabetes.