Association between polymorphisms of VKORC1 and CYP2C9 genes with warfarin maintenance dose in a group of warfarin users in Birjand city, Iran

• Published in: Journal of cellular biochemistry 2019-06, Vol.120 (6), p.9588-9593
• Main Authors: Fereidouni, Mohammad, Moossavi, Maryam, Kazemi, Touba, Nouranihassankiade, Shaghayeghsadat, Asghari, Arghavan
• Format: Article
• Language: English
• Subjects: Anticoagulants; Bleeding; CYP2C9; CYP2C9 gene; CYP450; dosage; Females; Gene polymorphism; Maintenance; Males; Patients; Polymerase chain reaction; Polymorphism; polymorphisms; Restriction fragment length polymorphism; Statistical analysis; Stochasticity; VKORC; Warfarin
• ISSN: 0730-2312; 1097-4644
• DOI: 10.1002/jcb.28235

Warfarin is the cardinal anticoagulant drug prescribed around the world. Due to stochastic bleeding in patients, it is essential to adjust the dose for every individual. The aim of the present study was to evaluate the frequency of CYP2C9 and VKORC1 gene polymorphisms and their association with warfarin maintenance dose in a sample of cardiovascular patients in Birjand, South‐Khorasan province of Iran. Patients with a history of cardiovascular disorders who take warfarin daily were selected. CYP2C9 and VKORC1 gene polymorphisms were detected by polymerase chain reaction‐restriction fragment length polymorphism in all participants. A total of 114 patients (mean age: 52.7 ± 14.9 years, M/F ratio: 0.76) participated in this study. Regarding CYP2C9 gene polymorphisms, the most frequent genotype was 1*/1* (80.4% in females and 62.5% in males). The frequency of 1*/2* and 2*/2* variants was 13% and 6.5% in females and 25% and 12.5% in males, respectively. The frequency of VKORC1 gene (1639 G > A), was 31.5%, 39.5%, and 29% for GG, GA, and AA in males, respectively. Besides, the mentioned genotype frequencies for females were 50%, 40.5%, and 9.5%, respectively. Moreover, there was a statistically significant correlation between VKORC1 gene −1639 G > A variant and warfarin maintenance dose (P