Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates

Aims Lignocellulosic biomass deconstruction is a bottleneck for obtaining biofuels and value‐added products. Our main goal was to characterize the secretome of a novel isolate, Cellulomonas sp. B6, when grown on residual biomass for the formulation of cost‐efficient enzymatic cocktails. Methods and...

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Bibliographic Details
Published in:Journal of applied microbiology 2019-03, Vol.126 (3), p.811-825, Article jam.14176
Main Authors: Piccinni, F.E., Ontañon, O.M., Ghio, S., Sauka, D.H., Talia, P.M., Rivarola, M.L., Valacco, M.P., Campos, E.
Format: Article
Language:English
Subjects:
Arabinofuranosidase
Biofuels
Biomass
Bioprocessing
Carboxymethylcellulose
cazymes
cellulomonas
Deconstruction
Extrusion
Genomes
gh10
glucanases
Glucosidase
Glycoside hydrolase
glycosyl hydrolases
Identification methods
Lignocellulose
Proteins
Secretion
Secretome
Straw
Substrates
Sugar
Sugarcane
Wheat
xylanases
Xyloglucanase
Xylosidase
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Summary:Aims Lignocellulosic biomass deconstruction is a bottleneck for obtaining biofuels and value‐added products. Our main goal was to characterize the secretome of a novel isolate, Cellulomonas sp. B6, when grown on residual biomass for the formulation of cost‐efficient enzymatic cocktails. Methods and Results We identified 205 potential CAZymes in the genome of Cellulomonas sp. B6, 91 of which were glycoside hydrolases (GH). By secretome analysis of supernatants from cultures in either extruded wheat straw (EWS), grinded sugar cane straw (SCR) or carboxymethylcellulose (CMC), we identified which proteins played a role in lignocellulose deconstruction. Growth on CMC resulted in the secretion of two exoglucanases (GH6 and GH48) and two GH10 xylanases, while growth on SCR or EWS resulted in the identification of a diversity of CAZymes. From the 32 GHs predicted to be secreted, 22 were identified in supernatants from EWS and/or SCR cultures, including endo‐ and exoglucanases, xylanases, a xyloglucanase, an arabinofuranosidase/β‐xylosidase, a β‐glucosidase and an AA10. Surprisingly, among the xylanases, seven were GH10. Conclusions Growth of Cellulomonas sp. B6 on lignocellulosic biomass induced the secretion of a diverse repertoire of CAZymes. Significance and Impact of the Study Cellulomonas sp. B6 could serve as a source of lignocellulose‐degrading enzymes applicable to bioprocessing and biotechnological industries.
ISSN:1364-5072
1365-2672
DOI:10.1111/jam.14176