Scutellarein inhibits RANKL‐induced osteoclast formation in vitro and prevents LPS‐induced bone loss in vivo

• Published in: Journal of cellular physiology 2019-07, Vol.234 (7), p.11951-11959
• Main Authors: Fu, Fangsheng, Shao, Siyuan, Wang, Ziyi, Song, Fangming, Lin, Xixi, Ding, Jiaxin, Li, Chen, Wu, Zuoxing, Li, Kai, Xiao, Yu, Su, Yiji, Zhao, Jinmin, Liu, Qian, Xu, Jiake
• Format: Article
• Language: English
• Subjects: Animals; Apigenin - pharmacology; Arthritis; Biocompatibility; Biomedical materials; Bone Diseases, Metabolic - metabolism; Bone implants; Bone loss; Bone resorption; Bone Resorption - drug therapy; Bone Resorption - pathology; Bone tumors; Cell Differentiation - drug effects; Flavonoids; Gelatinase B; Gene expression; In vivo methods and tests; Inflammation; Joint diseases; Lipopolysaccharides; Lipopolysaccharides - pharmacology; Loosening; Macrophages - metabolism; Mice, Inbred C57BL; Morphology; NF-AT protein; NFATC Transcription Factors - drug effects; NFATC Transcription Factors - metabolism; NFATc1; NF‐κB; osteoclast (OC); Osteoclastogenesis; Osteoclasts; Osteoclasts - drug effects; Osteoclasts - metabolism; Osteogenesis - drug effects; Osteolysis; Osteoporosis; scutellarein (Scu); TRANCE protein; Tumors
• ISSN: 0021-9541; 1097-4652
• DOI: 10.1002/jcp.27888

Osteoporosis, arthritis, Peget's disease, bone tumor, periprosthetic joint infection, and periprosthetic loosening have a common characteristic of osteolysis, which is characterized by the enhanced osteoclastic bone resorptive function. At present, the treatment target of these diseases is to interfere with osteoclastic formation and function. Scutellarein (Scu), a flavonoids compound, can inhibit the progress of tumor and inflammation. However, the role of Scu in inflammatory osteolysis isn’t elucidated clearly. Our study showed that Scu inhibited bone destruction induced by LPS in vivo and OC morphology and function induced by RANKL in vitro. Mechanistic studies revealed that Scu suppressed osteoclastic marker gene expression by RANKL‐induced, such as Ctsk9, Mmp9, Acp5, and Atp6v0d2. In addition, we found that the inhibition effects of osteoclastogenesis and bone resorption function of Scu were mediated via attenuating NF‐κB and NFAT signaling pathways. In conclusion, the results showed that Scu may become a potential new drug for the treatment of inflammatory osteolysis. Scutellarein could inhibit osteoclastogenesis and bone resorptive function via attenuating NF‐κB and NFAT signaling pathways. The results showed that Scu may become a potential new drug for the treatment of inflammatory osteolysis.