A fluorometric aptasensor for bisphenol a based on the inner filter effect of gold nanoparticles on the fluorescence of nitrogen-doped carbon dots

An aptamer-based fluorometric assay is described for the determination of bisphenol A (BPA). The aptamer against BPA is first attached to the surface of the red AuNPs, and this prevents the AuNPs from salt-induced formation of a blue-colored aggregate. Hence, the blue fluorescence of added nitrogen-...

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Bibliographic Details
Published in:Mikrochimica acta (1966) 2019-01, Vol.186 (1), p.28-9, Article 28
Main Authors: Wang, Li, Cao, Hai-Xia, Pan, Chang-Gang, He, Yu-Sheng, Liu, Hong-Fei, Zhou, Li-Hong, Li, Cai-Qi, Liang, Guo-Xi
Format: Article
Language:English
Subjects:
Analytical Chemistry
Bisphenol A
Carbon dots
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Drinking water
Fluorescence
Gold
Microengineering
Nanochemistry
Nanoparticles
Nanotechnology
Original Paper
Phenols
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Summary:An aptamer-based fluorometric assay is described for the determination of bisphenol A (BPA). The aptamer against BPA is first attached to the surface of the red AuNPs, and this prevents the AuNPs from salt-induced formation of a blue-colored aggregate. Hence, the blue fluorescence of added nitrogen-doped carbon dots (NCDots) is quenched via an inner filter effect (IFE) caused by the red AuNPs. After addition of BPA, the BPA/aptamer complex is formed, and the AuNPs are no longer stabilized agains aggregation. This weakens the IFE and results in the recovery of the fluorescence of the NCDots which is measured best at excitation/emission wavelengths of 300/420 nm. The recovered fluorescence increases linearly in the 10 to 250 nM and 250 to 900 nM BPA concentration ranges, and the detection limit is 3.3 nM. The method was successfully applied to the determination of BPA in spiked environmental tap water samples. Graphical abstract Schematic presentation of a fluorometric aptamer based assay for bisphenol A (BPA). It is based on the inner filter effect of gold nanoparticles (AuNPs) on the fluorescence of nitrogen-doped carbon dots (NCDots).
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-018-3153-3